| pubmed-article:9212051 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9212051 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:9212051 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:9212051 | lifeskim:mentions | umls-concept:C0205245 | lld:lifeskim |
| pubmed-article:9212051 | lifeskim:mentions | umls-concept:C0015295 | lld:lifeskim |
| pubmed-article:9212051 | lifeskim:mentions | umls-concept:C1413767 | lld:lifeskim |
| pubmed-article:9212051 | lifeskim:mentions | umls-concept:C1456379 | lld:lifeskim |
| pubmed-article:9212051 | lifeskim:mentions | umls-concept:C1519249 | lld:lifeskim |
| pubmed-article:9212051 | lifeskim:mentions | umls-concept:C0013879 | lld:lifeskim |
| pubmed-article:9212051 | lifeskim:mentions | umls-concept:C2350180 | lld:lifeskim |
| pubmed-article:9212051 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:9212051 | pubmed:dateCreated | 1997-9-2 | lld:pubmed |
| pubmed-article:9212051 | pubmed:abstractText | Two hormone-responsive segments, one in the region of the promoter and one in intron 1, are identified in two homologous androgen-regulated and differentially expressed rat genes encoding the cystatin-related proteins (CRPs). Footprint analysis with the androgen receptor (AR) DNA-binding domain on the promoter-containing fragments reveals an AR-binding site downstream of the transcription start point in the crp2 gene (ARBSd/crp2, +40/+63). It displays an androgen response element-like sequence motif 5'-AGAAGAaaaTGTACA-3' and overlaps with the ATG translation start codon. A double-stranded oligonucleotide containing this sequence forms a DNA-protein complex with the full-length AR synthesized by vaccinia, as seen in band shift assays. Additional AR-binding sites, ARBSu/crp1 and ARBSu/crp2, occur 5' upstream of the transcription start point and are located at an identical position (-142/ -120) in crp1 and crp2. The AR affinity for these two slightly different sequence motifs is relatively weak. The biological function of all three AR-binding sites as transcription control elements has been studied. The ARBSd/crp2 element clearly shows androgen-response element characteristics. The contribution of the common upstream element to the androgen-dependent control of reporter gene transcription is less clear. The transcription of a reporter gene construct containing the crp2 footprint fragment crp2F (-273/+88) is hormonally regulated as determined by transfection into the human breast cancer cell line T-47D. Androgens, but also glucocorticoids, efficiently stimulate steroid-dependent transcription of the chloramphenicol acetyltransferase gene. Mutation of the 5'-TGTACA-3' sequence in ARBSd/crp2 destroys the AR binding and abolishes the androgen-dependent synthesis of chloramphenicol acetyltransferase. A large fragment derived from intron 1 of the crp1 and crp2 gene can also provide the androgen-dependent transcription of chimeric constructs in T-47D cells. However, the induction measured is less than the one observed with crp2F (-273/+88), and this activity seems to reside in several subfragments that each display a low but consistent androgen responsiveness. | lld:pubmed |
| pubmed-article:9212051 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9212051 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9212051 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9212051 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9212051 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:9212051 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9212051 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9212051 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9212051 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9212051 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9212051 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:9212051 | pubmed:issn | 0888-8809 | lld:pubmed |
| pubmed-article:9212051 | pubmed:author | pubmed-author:HeynsWW | lld:pubmed |
| pubmed-article:9212051 | pubmed:author | pubmed-author:PeetersBB | lld:pubmed |
| pubmed-article:9212051 | pubmed:author | pubmed-author:RombautsWW | lld:pubmed |
| pubmed-article:9212051 | pubmed:author | pubmed-author:AldyDD | lld:pubmed |
| pubmed-article:9212051 | pubmed:author | pubmed-author:DevosAA | lld:pubmed |
| pubmed-article:9212051 | pubmed:author | pubmed-author:WinderickxJJ | lld:pubmed |
| pubmed-article:9212051 | pubmed:author | pubmed-author:ClaessensFF | lld:pubmed |
| pubmed-article:9212051 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9212051 | pubmed:volume | 11 | lld:pubmed |
| pubmed-article:9212051 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9212051 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9212051 | pubmed:pagination | 1033-43 | lld:pubmed |
| pubmed-article:9212051 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:9212051 | pubmed:meshHeading | pubmed-meshheading:9212051-... | lld:pubmed |
| pubmed-article:9212051 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9212051 | pubmed:articleTitle | Identification of a functional androgen-response element in the exon 1-coding sequence of the cystatin-related protein gene crp2. | lld:pubmed |
| pubmed-article:9212051 | pubmed:affiliation | Division of Biochemistry, Faculty of Medicine, University of Leuven, Belgium. | lld:pubmed |
| pubmed-article:9212051 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9212051 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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