| pubmed-article:8977194 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8977194 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:8977194 | lifeskim:mentions | umls-concept:C0014597 | lld:lifeskim |
| pubmed-article:8977194 | lifeskim:mentions | umls-concept:C0126732 | lld:lifeskim |
| pubmed-article:8977194 | lifeskim:mentions | umls-concept:C0009368 | lld:lifeskim |
| pubmed-article:8977194 | lifeskim:mentions | umls-concept:C0699900 | lld:lifeskim |
| pubmed-article:8977194 | lifeskim:mentions | umls-concept:C0243125 | lld:lifeskim |
| pubmed-article:8977194 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:8977194 | lifeskim:mentions | umls-concept:C0205349 | lld:lifeskim |
| pubmed-article:8977194 | lifeskim:mentions | umls-concept:C0332120 | lld:lifeskim |
| pubmed-article:8977194 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:8977194 | pubmed:dateCreated | 1997-1-30 | lld:pubmed |
| pubmed-article:8977194 | pubmed:abstractText | The nuclear factor kappaB (NF-kappaB) regulates the transcription of genes bearing the kappaB consensus motif. Transmigration of NF-kappaB from the cytoplasm to the nucleus is regulated by the IkappaB family of inhibitory NF-kappaB-binding proteins. Dissociation of the NF-kappaB-IkappaB complex requires both phosphorylation and degradation of IkappaBs. We demonstrate that IL-1beta induces complete IkappaB alpha degradation in Caco-2 cell lines but not in HT-29, T84, SW-480 transformed cell lines, or native colonic epithelial cells. A similar lack of IkappaB alpha degradation in HT-29 cells followed TNF-alpha and bacterial polymer stimulation. IL-1beta stimulated NF-kappaB nuclear translocation and NF-kappaB-dependent IL-1beta and IL-8 expression in both Caco-2 and HT-29 cells as assayed by electrophoretic mobility shift assay, immunofluorescence, kappaB-luciferase transfection, reverse transcriptase-PCR analysis and ELISA. In HT-29 cells stimulated with IL-1beta, IkappaB alpha was phosphorylated and when cycloheximide blocked new protein synthesis, IkappaB alpha was partially degraded. NF-kappaB cytoplasmic to nuclear transmigration was incomplete and preceded IkappaB alpha degradation in 9T-29 cells, in contrast to complete coordinated NF-kappaB nuclear translocation and IkappaB alpha degradation in Caco-2 cells. Greater sensitivity of HT-29 cells to a calpain inhibitor, as measured by IL-8 secretion, suggested enhanced resistance to IkappaB alpha proteolysis. These data show that IL-1beta induces NF-kappaB activity and expression of NF-kappaB-dependent genes in colonic epithelial cells and suggest altered regulation of IkappaB alpha degradation compared with other cell lineages, which may result in their increased responsiveness to therapeutic blockade. | lld:pubmed |
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| pubmed-article:8977194 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8977194 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8977194 | pubmed:citationSubset | AIM | lld:pubmed |
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| pubmed-article:8977194 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8977194 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:8977194 | pubmed:issn | 0022-1767 | lld:pubmed |
| pubmed-article:8977194 | pubmed:author | pubmed-author:MayerLL | lld:pubmed |
| pubmed-article:8977194 | pubmed:author | pubmed-author:PanisRR | lld:pubmed |
| pubmed-article:8977194 | pubmed:author | pubmed-author:HaskillSS | lld:pubmed |
| pubmed-article:8977194 | pubmed:author | pubmed-author:SartorR BRB | lld:pubmed |
| pubmed-article:8977194 | pubmed:author | pubmed-author:JobinCC | lld:pubmed |
| pubmed-article:8977194 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8977194 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:8977194 | pubmed:volume | 158 | lld:pubmed |
| pubmed-article:8977194 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8977194 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8977194 | pubmed:pagination | 226-34 | lld:pubmed |
| pubmed-article:8977194 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:8977194 | pubmed:meshHeading | pubmed-meshheading:8977194-... | lld:pubmed |
| pubmed-article:8977194 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:8977194 | pubmed:articleTitle | Evidence for altered regulation of I kappa B alpha degradation in human colonic epithelial cells. | lld:pubmed |
| pubmed-article:8977194 | pubmed:affiliation | Department of Medicine, University of North Carolina at Chapel Hill, 27599, USA. | lld:pubmed |
| pubmed-article:8977194 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8977194 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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