| pubmed-article:8954765 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8954765 | lifeskim:mentions | umls-concept:C0162641 | lld:lifeskim |
| pubmed-article:8954765 | lifeskim:mentions | umls-concept:C0162642 | lld:lifeskim |
| pubmed-article:8954765 | lifeskim:mentions | umls-concept:C0002844 | lld:lifeskim |
| pubmed-article:8954765 | lifeskim:mentions | umls-concept:C0042896 | lld:lifeskim |
| pubmed-article:8954765 | lifeskim:mentions | umls-concept:C0021467 | lld:lifeskim |
| pubmed-article:8954765 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:8954765 | lifeskim:mentions | umls-concept:C0021469 | lld:lifeskim |
| pubmed-article:8954765 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:8954765 | pubmed:dateCreated | 1997-3-10 | lld:pubmed |
| pubmed-article:8954765 | pubmed:abstractText | Treatment of mature female tilapia (Oreochromis niloticus) with high levels of androgen (17 alpha-methyltestosterone, 17 alpha MT) results in a pronounced decline in plasma vitellogenin levels as determined by gel electrophoresis. Total RNA extracted from livers of treated fish and vehicle-injected controls was analyzed by Northern and slot blot hybridization using an oligonucleotide complementary to a sequence in the 3' end of tilapia vitellogenin mRNA. The probe revealed an mRNA of 6.5 kb in liver from the control mature female fish which was decreased by 85% by androgen treatment. As expected, estradiol (E2) treatment induced the 6.5-kb mRNA in mature male tilapia. The antiestrogen, tamoxifen, strongly decreased vitellogenin mRNA levels in mature females. Radioimmunoassay of serum from control and 17 alpha MT-treated female tilapia showed a marked reduction in serum E2 levels, from 11.4 +/- 2.6 ng/ml in controls to 2.2 +/- 0.13 ng/ml in treated fish. Tamoxifen, however, resulted in increased serum E2 levels, probably by blocking E2 negative feedback. The serum E2-lowering effect of 17 alpha MT suggests an inhibitory site of action on gonadotropin production at the hypothalamic-pituitary axis, possibly through an androgen receptor or through an estrogen receptor after local aromatization of 17 alpha MT. | lld:pubmed |
| pubmed-article:8954765 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8954765 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8954765 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8954765 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8954765 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8954765 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8954765 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8954765 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8954765 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8954765 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8954765 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8954765 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8954765 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:8954765 | pubmed:issn | 0016-6480 | lld:pubmed |
| pubmed-article:8954765 | pubmed:author | pubmed-author:WrightJ MJM | lld:pubmed |
| pubmed-article:8954765 | pubmed:author | pubmed-author:LazierC BCB | lld:pubmed |
| pubmed-article:8954765 | pubmed:author | pubmed-author:LangleySS | lld:pubmed |
| pubmed-article:8954765 | pubmed:author | pubmed-author:RamseyN BNB | lld:pubmed |
| pubmed-article:8954765 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8954765 | pubmed:volume | 104 | lld:pubmed |
| pubmed-article:8954765 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8954765 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8954765 | pubmed:pagination | 321-9 | lld:pubmed |
| pubmed-article:8954765 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:8954765 | pubmed:meshHeading | pubmed-meshheading:8954765-... | lld:pubmed |
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| pubmed-article:8954765 | pubmed:meshHeading | pubmed-meshheading:8954765-... | lld:pubmed |
| pubmed-article:8954765 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8954765 | pubmed:articleTitle | Androgen inhibition of vitellogenin gene expression in tilapia (Oreochromis niloticus). | lld:pubmed |
| pubmed-article:8954765 | pubmed:affiliation | Department of Biochemistry, Dalhousie University, Halifax, Nova Scotia, Canada. CBLAZIE@AC.DAL.CA | lld:pubmed |
| pubmed-article:8954765 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8954765 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
