| pubmed-article:8952702 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8952702 | lifeskim:mentions | umls-concept:C0018557 | lld:lifeskim |
| pubmed-article:8952702 | lifeskim:mentions | umls-concept:C0028128 | lld:lifeskim |
| pubmed-article:8952702 | lifeskim:mentions | umls-concept:C0016030 | lld:lifeskim |
| pubmed-article:8952702 | lifeskim:mentions | umls-concept:C1510411 | lld:lifeskim |
| pubmed-article:8952702 | lifeskim:mentions | umls-concept:C0021755 | lld:lifeskim |
| pubmed-article:8952702 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:8952702 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:8952702 | lifeskim:mentions | umls-concept:C1546857 | lld:lifeskim |
| pubmed-article:8952702 | lifeskim:mentions | umls-concept:C1556066 | lld:lifeskim |
| pubmed-article:8952702 | lifeskim:mentions | umls-concept:C1619636 | lld:lifeskim |
| pubmed-article:8952702 | lifeskim:mentions | umls-concept:C1514873 | lld:lifeskim |
| pubmed-article:8952702 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:8952702 | pubmed:dateCreated | 1997-1-7 | lld:pubmed |
| pubmed-article:8952702 | pubmed:abstractText | Interleukin-1 (IL-1) is known to inhibit proliferation in some tumor cells. This proinflammatory cytokine also induces nitric oxide production in a variety of cell types. In the present studies we determined if nitric oxide is involved in IL-1 induced growth inhibition in spontaneously transformed hamster embryonic fibroblasts (STHE cells). Both IL-1 alpha and IL-1 beta were found to stimulate nitric oxide production and to reduce 3H-thymidine (TdR) incorporation in high density cultures of STHE cells. However, maximal cytostasis was observed at least 24 h before significant amounts of nitric oxide accumulated in the cultures. In addition, doses of IL-1 which were too low to stimulate nitric oxide synthesis were effective in inducing cytostasis. Furthermore, in low density cultures of STHE cells, IL-1 inhibited DNA synthesis without inducing nitric oxide production. The nitric oxide synthase inhibitor NG-monomethyl-1-arginine (L-NMMA) had no effect on proliferation of cells plated at low density. In contrast, L-NMMA treatment resulted in a 40-60% reduction in IL-1 induced cytostasis in high density cultures. Neutralizing antibodies to IL-1 were found to completely block IL-1 induced cytostasis and nitric oxide production in cells plated at both densities. Although anti-IL-1 alpha and anti-IL-1 beta antibodies were highly specific and did not cross react, anti-tumor necrosis factor-alpha (TNF-alpha) antibody was able to partially suppress activation of STHE cells by both IL-1 alpha and IL-1 beta. These data suggest a potential involvement of endogenous TNF-alpha in IL-1 induced cytostasis and nitric oxide production. Exponentially growing STHE cells produced six-times less nitric oxide than non-proliferating cells. A ten-fold excess of 1-arginine was found to stimulate nitric oxide synthesis, an action that was independent of the rate of cellular proliferation. Taken together these data suggest that nitric oxide is not a major mediator of IL-1 induced cytostasis in STHE cells. Moreover, cytostasis appears to be required for nitric oxide synthesis in these cells. | lld:pubmed |
| pubmed-article:8952702 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8952702 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8952702 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8952702 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8952702 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8952702 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8952702 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8952702 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8952702 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8952702 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8952702 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8952702 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8952702 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8952702 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8952702 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:8952702 | pubmed:issn | 0021-9541 | lld:pubmed |
| pubmed-article:8952702 | pubmed:author | pubmed-author:PatelNN | lld:pubmed |
| pubmed-article:8952702 | pubmed:author | pubmed-author:LaskinD LDL | lld:pubmed |
| pubmed-article:8952702 | pubmed:author | pubmed-author:LavnikovaNN | lld:pubmed |
| pubmed-article:8952702 | pubmed:author | pubmed-author:ProkhorovaSS | lld:pubmed |
| pubmed-article:8952702 | pubmed:author | pubmed-author:LakhotiaAA | lld:pubmed |
| pubmed-article:8952702 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8952702 | pubmed:volume | 169 | lld:pubmed |
| pubmed-article:8952702 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8952702 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8952702 | pubmed:pagination | 532-7 | lld:pubmed |
| pubmed-article:8952702 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:8952702 | pubmed:meshHeading | pubmed-meshheading:8952702-... | lld:pubmed |
| pubmed-article:8952702 | pubmed:meshHeading | pubmed-meshheading:8952702-... | lld:pubmed |
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| pubmed-article:8952702 | pubmed:meshHeading | pubmed-meshheading:8952702-... | lld:pubmed |
| pubmed-article:8952702 | pubmed:meshHeading | pubmed-meshheading:8952702-... | lld:pubmed |
| pubmed-article:8952702 | pubmed:meshHeading | pubmed-meshheading:8952702-... | lld:pubmed |
| pubmed-article:8952702 | pubmed:meshHeading | pubmed-meshheading:8952702-... | lld:pubmed |
| pubmed-article:8952702 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8952702 | pubmed:articleTitle | Cytostasis is required for IL-1 induced nitric oxide production in transformed hamster fibroblasts. | lld:pubmed |
| pubmed-article:8952702 | pubmed:affiliation | Rutgers University, Piscataway, New Jersey 08855-0789, USA. | lld:pubmed |
| pubmed-article:8952702 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8952702 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8952702 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8952702 | lld:pubmed |
