| pubmed-article:8951229 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8951229 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
| pubmed-article:8951229 | lifeskim:mentions | umls-concept:C0039808 | lld:lifeskim |
| pubmed-article:8951229 | lifeskim:mentions | umls-concept:C1883254 | lld:lifeskim |
| pubmed-article:8951229 | lifeskim:mentions | umls-concept:C0205360 | lld:lifeskim |
| pubmed-article:8951229 | lifeskim:mentions | umls-concept:C0332256 | lld:lifeskim |
| pubmed-article:8951229 | lifeskim:mentions | umls-concept:C0037791 | lld:lifeskim |
| pubmed-article:8951229 | lifeskim:mentions | umls-concept:C0536131 | lld:lifeskim |
| pubmed-article:8951229 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:8951229 | pubmed:dateCreated | 1997-3-17 | lld:pubmed |
| pubmed-article:8951229 | pubmed:abstractText | 8-Methyl-2'-deoxyguanosine (8-MedG) was synthesized by reacting dG under the methyl radical generating system and incorporated into oligodeoxynucleotides using phosphoramidite techniques. The site-specifically modified oligodeoxynucleotide containing a single 8-MedG was then used as a template for primer extension reactions catalyzed by the 3' --> 5' exonuclease-free (exo-) Klenow fragment of Escherichia Coli DNA polymerase I and mammalian DNA polymerase alpha. Primer extension catalyzed by the exo- Klenow fragment readily passed the 8-MedG lesion in the template while that catalyzed by pol alpha was retarded opposite the lesion. The fully extended products formed during DNA synthesis were analyzed to quantify the miscoding specificities of 8-MedG. Both DNA polymerases incorporated primarily dCMP, the correct base opposite the lesion, along with small amounts of incorporation of dGMP and dAMP. In addition, two-base deletion was observed only when the exo- Klenow fragment was used. The thermodynamic stability of 8-MedG in the duplex was also studied. The duplex containing 8-MedG:dG was more thermally and thermodynamically stable than that of dG:dG. The duplex containing 8-MedG:dA was more thermodynamically stable than that of dG:dA. We conclude that 8-MedG is a miscoding lesion and capable of generating G --> C and G --> T transversions and deletion in cells. | lld:pubmed |
| pubmed-article:8951229 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8951229 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8951229 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8951229 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8951229 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8951229 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8951229 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8951229 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8951229 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:8951229 | pubmed:issn | 0893-228X | lld:pubmed |
| pubmed-article:8951229 | pubmed:author | pubmed-author:TanabeKK | lld:pubmed |
| pubmed-article:8951229 | pubmed:author | pubmed-author:ShibutaniSS | lld:pubmed |
| pubmed-article:8951229 | pubmed:author | pubmed-author:KohdaKK | lld:pubmed |
| pubmed-article:8951229 | pubmed:author | pubmed-author:MinouraYY | lld:pubmed |
| pubmed-article:8951229 | pubmed:author | pubmed-author:TsunomotoHH | lld:pubmed |
| pubmed-article:8951229 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8951229 | pubmed:volume | 9 | lld:pubmed |
| pubmed-article:8951229 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8951229 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8951229 | pubmed:pagination | 1278-84 | lld:pubmed |
| pubmed-article:8951229 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:8951229 | pubmed:meshHeading | pubmed-meshheading:8951229-... | lld:pubmed |
| pubmed-article:8951229 | pubmed:meshHeading | pubmed-meshheading:8951229-... | lld:pubmed |
| pubmed-article:8951229 | pubmed:meshHeading | pubmed-meshheading:8951229-... | lld:pubmed |
| pubmed-article:8951229 | pubmed:meshHeading | pubmed-meshheading:8951229-... | lld:pubmed |
| pubmed-article:8951229 | pubmed:meshHeading | pubmed-meshheading:8951229-... | lld:pubmed |
| pubmed-article:8951229 | pubmed:meshHeading | pubmed-meshheading:8951229-... | lld:pubmed |
| pubmed-article:8951229 | pubmed:meshHeading | pubmed-meshheading:8951229-... | lld:pubmed |
| pubmed-article:8951229 | pubmed:meshHeading | pubmed-meshheading:8951229-... | lld:pubmed |
| pubmed-article:8951229 | pubmed:meshHeading | pubmed-meshheading:8951229-... | lld:pubmed |
| pubmed-article:8951229 | pubmed:meshHeading | pubmed-meshheading:8951229-... | lld:pubmed |
| pubmed-article:8951229 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8951229 | pubmed:articleTitle | Synthesis, miscoding specificity, and thermodynamic stability of oligodeoxynucleotide containing 8-methyl-2'-deoxyguanosine. | lld:pubmed |
| pubmed-article:8951229 | pubmed:affiliation | Faculty of Pharmaceutical Sciences, Nagoya City University, Japan. kohda@phar.nagoya-cu.ac.jp | lld:pubmed |
| pubmed-article:8951229 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8951229 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8951229 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8951229 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8951229 | lld:pubmed |
