pubmed-article:8932396 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:8932396 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
pubmed-article:8932396 | lifeskim:mentions | umls-concept:C0035668 | lld:lifeskim |
pubmed-article:8932396 | lifeskim:mentions | umls-concept:C0205352 | lld:lifeskim |
pubmed-article:8932396 | pubmed:issue | 21 | lld:pubmed |
pubmed-article:8932396 | pubmed:dateCreated | 1996-12-20 | lld:pubmed |
pubmed-article:8932396 | pubmed:abstractText | We describe a simple method for 3'-end labeling RNAs of known sequence. A short DNA template is designed to anneal to the 3'-end of the RNA, with a two nucleotide 5' overhang of 3'-TA-5', 3'-TG-5' or 3'-TC-5'. The Klenow fragment of DNA polymerase I can then cleanly and efficiently extend the 3'-end of the RNA by the incorporation of a single alpha-32P-labeled dATP residue. This method can be used to label one RNA in a mixture of RNAs, or to label 5'-blocked RNAs such as mRNA. | lld:pubmed |
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pubmed-article:8932396 | pubmed:language | eng | lld:pubmed |
pubmed-article:8932396 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8932396 | pubmed:citationSubset | IM | lld:pubmed |
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pubmed-article:8932396 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:8932396 | pubmed:month | Nov | lld:pubmed |
pubmed-article:8932396 | pubmed:issn | 0305-1048 | lld:pubmed |
pubmed-article:8932396 | pubmed:author | pubmed-author:SzostakJ WJW | lld:pubmed |
pubmed-article:8932396 | pubmed:author | pubmed-author:HuangZZ | lld:pubmed |
pubmed-article:8932396 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:8932396 | pubmed:day | 1 | lld:pubmed |
pubmed-article:8932396 | pubmed:volume | 24 | lld:pubmed |
pubmed-article:8932396 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:8932396 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:8932396 | pubmed:pagination | 4360-1 | lld:pubmed |
pubmed-article:8932396 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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pubmed-article:8932396 | pubmed:year | 1996 | lld:pubmed |
pubmed-article:8932396 | pubmed:articleTitle | A simple method for 3'-labeling of RNA. | lld:pubmed |
pubmed-article:8932396 | pubmed:affiliation | Department of Molecular Biology, Massachusetts General Hospital, Boston 02114, USA. | lld:pubmed |
pubmed-article:8932396 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:8932396 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
pubmed-article:8932396 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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