Linked Life Data

8928790

Source:http://linkedlifedata.com/resource/pubmed/id/8928790

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pubmed-article:8928790pubmed:abstractTextBinding and localization of transforming growth factor-alpha (TGF-alpha) and epidermal growth factor (EGF) were assessed using in vitro primary cultures of guinea pig gastric mucous epithelial cells (GMEC). GMEC were isolated and cultured in six-well plates with Dulbecco's modified Eagle's medium + 10% serum and then changed to serum-free medium for 24 h for binding studies. The binding time course of 125I-labeled EGF and 125I-TGF-alpha in GMEC cultures at 4 degrees C was saturable, reaching a plateau within 4-6 h. Competition-binding curves revealed that the amount of unlabeled EGF and TGF-alpha to reduce 125I-EGF binding by 50% was 0.35 and 0.23 nM, respectively. The amount of unlabeled EGF and TGF-alpha to decrease 125I-TGF-alpha binding by 50% was 0.30 and 0.21 nM, respectively. A Scatchard analysis of the data disclosed that a single class of high-affinity binding sites (dissociation constant = 0.24 nM) was present. The maximal binding capacity was approximately 20 fmol/10(6) cells or approximately 12,000 receptors per cell. The binding of 125I-EGF and 125I-TFG-alpha to GMEC cultures was maximal between pH 7.0 and 8.5. No specific binding of EGF or TGF-alpha could be detected below pH 5.0. The half-maximal pH dissociation value for EGF and TGF-alpha was pH 5.89 and pH 6.83, respectively. We found no difference in the final amounts of membrane-bound or internalized 125I-EGF and 125I-TGF-alpha. However, there was a significant difference (P < 0.05) at 5-30 min in the rate of dissociated and internalized 125I-EGF- and 125I-TGF-alpha. Immunofluorescence microscopy of GMEC cultures for EGF/TGF-alpha receptors showed increased fluorescence at the leading edges and around the perimeter of cells. Detection of an EGF/TGF-alpha receptor was also confirmed by Western blotting. Our findings demonstrate that guinea pig GMEC possess a specific EGF/TGF-alpha receptor, which further supports a physiological role for EFG and TFG-alpha as mitogens in these cells.lld:pubmed
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pubmed-article:8928790pubmed:authorpubmed-author:DempseyP JPJlld:pubmed
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pubmed-article:8928790pubmed:authorpubmed-author:HawkeyM AMAlld:pubmed
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pubmed-article:8928790pubmed:paginationG604-12lld:pubmed
pubmed-article:8928790pubmed:dateRevised2009-11-19lld:pubmed
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pubmed-article:8928790pubmed:year1996lld:pubmed
pubmed-article:8928790pubmed:articleTitleIdentification of an EGF/TGF-alpha receptor in primary cultures of guinea pig gastric mucous epithelial cells.lld:pubmed
pubmed-article:8928790pubmed:affiliationDepartment of Surgery, Oregon Health Sciences University, Portland, USA.lld:pubmed
pubmed-article:8928790pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:8928790pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
pubmed-article:8928790pubmed:publicationTypeResearch Support, U.S. Gov't, Non-P.H.S.lld:pubmed
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