| pubmed-article:8910367 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8910367 | lifeskim:mentions | umls-concept:C0006675 | lld:lifeskim |
| pubmed-article:8910367 | lifeskim:mentions | umls-concept:C0162638 | lld:lifeskim |
| pubmed-article:8910367 | lifeskim:mentions | umls-concept:C0178719 | lld:lifeskim |
| pubmed-article:8910367 | lifeskim:mentions | umls-concept:C1545588 | lld:lifeskim |
| pubmed-article:8910367 | lifeskim:mentions | umls-concept:C1698986 | lld:lifeskim |
| pubmed-article:8910367 | lifeskim:mentions | umls-concept:C1611645 | lld:lifeskim |
| pubmed-article:8910367 | pubmed:issue | 44 | lld:pubmed |
| pubmed-article:8910367 | pubmed:dateCreated | 1996-12-26 | lld:pubmed |
| pubmed-article:8910367 | pubmed:abstractText | The ability of Bcl-2 to inhibit cell death is well documented but its mechanism of action remains elusive. Recent reports have suggested that Bcl-2 prevents apoptosis by inhibiting the release of Ca2+ from the thapsigargin-sensitive Ca2+ store. The mobilization of Ca2+ from this store has been implicated as a signal regulating apoptotic cell death induced by glucocorticoids and by interleukin-3 withdrawal. The present study was designed to determine if Bcl-2 would still inhibit apoptosis after depletion of intracellular Ca2+ stores. We compared the response of two Chinese hamster ovary cell lines (5AHSmyc and 5A300bcl-2.2) following incubation with the calcium ionophore ionomycin to deplete intracellular Ca2+ stores. Continued incubation of 5AHSmyc cells in calcium-free media induced substantial apoptotic DNA fragmentation within 4 h and >95% loss of viability within 48 h. However, 5A300bcl-2.2 cells showed no evidence of DNA fragmentation or loss of viability over the same time period. Intracellular Ca2+ was analyzed with the Ca2+-sensitive fluorescent dye INDO-1 and confirmed that ionomycin was capable of releasing Ca2+ from intracellular stores in both cell lines. These results show that depletion of intracellular Ca2+ stores induces apoptosis and that these Ca2+ stores are not required for the protection afforded by Bcl-2. | lld:pubmed |
| pubmed-article:8910367 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8910367 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8910367 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8910367 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8910367 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8910367 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8910367 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8910367 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8910367 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8910367 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8910367 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8910367 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8910367 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:8910367 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:8910367 | pubmed:author | pubmed-author:EastmanAA | lld:pubmed |
| pubmed-article:8910367 | pubmed:author | pubmed-author:ReynoldsJ EJE | lld:pubmed |
| pubmed-article:8910367 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8910367 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:8910367 | pubmed:volume | 271 | lld:pubmed |
| pubmed-article:8910367 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8910367 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8910367 | pubmed:pagination | 27739-43 | lld:pubmed |
| pubmed-article:8910367 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:8910367 | pubmed:meshHeading | pubmed-meshheading:8910367-... | lld:pubmed |
| pubmed-article:8910367 | pubmed:meshHeading | pubmed-meshheading:8910367-... | lld:pubmed |
| pubmed-article:8910367 | pubmed:meshHeading | pubmed-meshheading:8910367-... | lld:pubmed |
| pubmed-article:8910367 | pubmed:meshHeading | pubmed-meshheading:8910367-... | lld:pubmed |
| pubmed-article:8910367 | pubmed:meshHeading | pubmed-meshheading:8910367-... | lld:pubmed |
| pubmed-article:8910367 | pubmed:meshHeading | pubmed-meshheading:8910367-... | lld:pubmed |
| pubmed-article:8910367 | pubmed:meshHeading | pubmed-meshheading:8910367-... | lld:pubmed |
| pubmed-article:8910367 | pubmed:meshHeading | pubmed-meshheading:8910367-... | lld:pubmed |
| pubmed-article:8910367 | pubmed:meshHeading | pubmed-meshheading:8910367-... | lld:pubmed |
| pubmed-article:8910367 | pubmed:meshHeading | pubmed-meshheading:8910367-... | lld:pubmed |
| pubmed-article:8910367 | pubmed:meshHeading | pubmed-meshheading:8910367-... | lld:pubmed |
| pubmed-article:8910367 | pubmed:meshHeading | pubmed-meshheading:8910367-... | lld:pubmed |
| pubmed-article:8910367 | pubmed:meshHeading | pubmed-meshheading:8910367-... | lld:pubmed |
| pubmed-article:8910367 | pubmed:meshHeading | pubmed-meshheading:8910367-... | lld:pubmed |
| pubmed-article:8910367 | pubmed:meshHeading | pubmed-meshheading:8910367-... | lld:pubmed |
| pubmed-article:8910367 | pubmed:meshHeading | pubmed-meshheading:8910367-... | lld:pubmed |
| pubmed-article:8910367 | pubmed:meshHeading | pubmed-meshheading:8910367-... | lld:pubmed |
| pubmed-article:8910367 | pubmed:meshHeading | pubmed-meshheading:8910367-... | lld:pubmed |
| pubmed-article:8910367 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8910367 | pubmed:articleTitle | Intracellular calcium stores are not required for Bcl-2-mediated protection from apoptosis. | lld:pubmed |
| pubmed-article:8910367 | pubmed:affiliation | Department of Pharmacology, Dartmouth Medical School, Hanover, New Hampshire 03755, USA. | lld:pubmed |
| pubmed-article:8910367 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8910367 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8910367 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8910367 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8910367 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8910367 | lld:pubmed |
