| pubmed-article:8895534 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8895534 | lifeskim:mentions | umls-concept:C0042721 | lld:lifeskim |
| pubmed-article:8895534 | lifeskim:mentions | umls-concept:C0030705 | lld:lifeskim |
| pubmed-article:8895534 | lifeskim:mentions | umls-concept:C0736268 | lld:lifeskim |
| pubmed-article:8895534 | lifeskim:mentions | umls-concept:C0009221 | lld:lifeskim |
| pubmed-article:8895534 | lifeskim:mentions | umls-concept:C0032520 | lld:lifeskim |
| pubmed-article:8895534 | lifeskim:mentions | umls-concept:C1366631 | lld:lifeskim |
| pubmed-article:8895534 | lifeskim:mentions | umls-concept:C0079419 | lld:lifeskim |
| pubmed-article:8895534 | lifeskim:mentions | umls-concept:C1705167 | lld:lifeskim |
| pubmed-article:8895534 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:8895534 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:8895534 | pubmed:dateCreated | 1996-12-13 | lld:pubmed |
| pubmed-article:8895534 | pubmed:abstractText | AGG to AGT mutations in codon 249 of the p53 tumor-suppressor gene are frequently observed in hepatocellular carcinomas (HCC) from areas where exposure to aflatoxin B1 (AFB) occurs. We developed a sensitive allele-specific polymerase chain reaction (AS-PCR) assay to detect this point mutation in non-neoplastic human liver tissues. Three oligonucleotide primers, 1 specific for the mutant allele and 2 specific for the wild-type allele were used. The mutant allele primer differed from the wild-type allele due to a G-to-T transversion in its terminal 3' nucleotide. The first stage involved amplification of exon 7 of p53 followed by a selective amplification of mutant codon 249 sequences. This method allowed for the detection of a mutant codon 249 allele in the presence of as many as 105 copies of the wild-type allele and was 100-fold more sensitive than the restriction fragment length polymorphism-PCR technique. We have applied this AS-PCR protocol to examine codon 249 AGT transversion in tumor and matched non-tumor liver samples from North American patients with hepatitis and from Mozambiquan patients exposed to AFB. Mutations were detected in 5 of 6 samples of non-neoplastic liver from Mozambiquan patients, all of whom were HBsAg- or HBcAg-positive and AFB-exposed. In contrast, no mutations were detected in non-neoplastic liver from North American patients with either HBV- or HCV-derived hepatitis and cirrhosis. This procedure is a simple and powerful approach for screening p53 codon 249 AGT mutation in heterogeneous non-neoplastic hepatocyte populations. | lld:pubmed |
| pubmed-article:8895534 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8895534 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8895534 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8895534 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8895534 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8895534 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8895534 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:8895534 | pubmed:issn | 0020-7136 | lld:pubmed |
| pubmed-article:8895534 | pubmed:author | pubmed-author:YamasakiHH | lld:pubmed |
| pubmed-article:8895534 | pubmed:author | pubmed-author:NakazawaHH | lld:pubmed |
| pubmed-article:8895534 | pubmed:author | pubmed-author:MarZhZh | lld:pubmed |
| pubmed-article:8895534 | pubmed:author | pubmed-author:CameronR GRG | lld:pubmed |
| pubmed-article:8895534 | pubmed:author | pubmed-author:BatistGG | lld:pubmed |
| pubmed-article:8895534 | pubmed:author | pubmed-author:Alaoui-Jamali... | lld:pubmed |
| pubmed-article:8895534 | pubmed:author | pubmed-author:KirbyG MGM | lld:pubmed |
| pubmed-article:8895534 | pubmed:author | pubmed-author:Fotouhi-Ardak... | lld:pubmed |
| pubmed-article:8895534 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8895534 | pubmed:day | 27 | lld:pubmed |
| pubmed-article:8895534 | pubmed:volume | 68 | lld:pubmed |
| pubmed-article:8895534 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8895534 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8895534 | pubmed:pagination | 21-5 | lld:pubmed |
| pubmed-article:8895534 | pubmed:dateRevised | 2007-7-24 | lld:pubmed |
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| pubmed-article:8895534 | pubmed:meshHeading | pubmed-meshheading:8895534-... | lld:pubmed |
| pubmed-article:8895534 | pubmed:meshHeading | pubmed-meshheading:8895534-... | lld:pubmed |
| pubmed-article:8895534 | pubmed:meshHeading | pubmed-meshheading:8895534-... | lld:pubmed |
| pubmed-article:8895534 | pubmed:meshHeading | pubmed-meshheading:8895534-... | lld:pubmed |
| pubmed-article:8895534 | pubmed:meshHeading | pubmed-meshheading:8895534-... | lld:pubmed |
| pubmed-article:8895534 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8895534 | pubmed:articleTitle | Allele-specific PCR analysis of p53 codon 249 AGT transversion in liver tissues from patients with viral hepatitis. | lld:pubmed |
| pubmed-article:8895534 | pubmed:affiliation | Lady Davis Institute for Medical Research and Jewish General Hospital, Department of Medicine, McGill University, Montreal, Canada. | lld:pubmed |
| pubmed-article:8895534 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8895534 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8895534 | lld:pubmed |
