| pubmed-article:8892915 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8892915 | lifeskim:mentions | umls-concept:C0010823 | lld:lifeskim |
| pubmed-article:8892915 | lifeskim:mentions | umls-concept:C0020971 | lld:lifeskim |
| pubmed-article:8892915 | lifeskim:mentions | umls-concept:C0877837 | lld:lifeskim |
| pubmed-article:8892915 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:8892915 | lifeskim:mentions | umls-concept:C1545588 | lld:lifeskim |
| pubmed-article:8892915 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:8892915 | pubmed:dateCreated | 1996-12-30 | lld:pubmed |
| pubmed-article:8892915 | pubmed:abstractText | The murine cytomegalovirus (MCMV) immediate-early gene 1 (IE1) encodes an 89-kDa phosphoprotein (pp89) which plays a key role in protecting BALB/c mice against the lethal effects of the MCMV infection. In this report, we have addressed the question of whether "naked DNA" vaccination with a eukaryotic expression vector (pcDNA-89) that contains the MCMV IE1 gene driven by a strong enhancer/promoter can confer protection. BALB/c mice were immunized intradermally with pcDNA-89 or with the plasmid backbone pcDNAI/Amp (pcDNA) and then challenged 2 weeks later with either a lethal or a sublethal intraperitoneal dose of the K181 strain of MCMV. Variable results were obtained for the individual experiments in which mice received a lethal challenge. In four separate trials, an average of 63% of the mice immunized with pcDNA-89 survived, compared with 18% of the mice immunized with pcDNA. However, in two other trials there was no specific protection. The results of experiments in which mice were injected with a sublethal dose of MCMV were more consistent, and significant decreases in viral titer in the spleen and salivary glands of pcDNA-89-immunized mice were observed, relative to controls. At the time of peak viral replication, titers in the spleens of immunized mice were reduced 18- to >63-fold, while those in the salivary gland were reduced approximately 24- to 48-fold. Although DNA immunization elicited only a low level of seroconversion in these mice, by 7 weeks postimmunization the mice had generated a cytotoxic T-lymphocyte response against pp89. These results suggest that DNA vaccination with selected CMV genes may provide a safe and efficient means of immunizing against CMV disease. | lld:pubmed |
| pubmed-article:8892915 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8892915 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8892915 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892915 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8892915 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:8892915 | pubmed:issn | 0022-538X | lld:pubmed |
| pubmed-article:8892915 | pubmed:author | pubmed-author:SpectorD HDH | lld:pubmed |
| pubmed-article:8892915 | pubmed:author | pubmed-author:CranmerL DLD | lld:pubmed |
| pubmed-article:8892915 | pubmed:author | pubmed-author:González... | lld:pubmed |
| pubmed-article:8892915 | pubmed:author | pubmed-author:MorelloC SCS | lld:pubmed |
| pubmed-article:8892915 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8892915 | pubmed:volume | 70 | lld:pubmed |
| pubmed-article:8892915 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8892915 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8892915 | pubmed:pagination | 7921-8 | lld:pubmed |
| pubmed-article:8892915 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:8892915 | pubmed:meshHeading | pubmed-meshheading:8892915-... | lld:pubmed |
| pubmed-article:8892915 | pubmed:meshHeading | pubmed-meshheading:8892915-... | lld:pubmed |
| pubmed-article:8892915 | pubmed:meshHeading | pubmed-meshheading:8892915-... | lld:pubmed |
| pubmed-article:8892915 | pubmed:meshHeading | pubmed-meshheading:8892915-... | lld:pubmed |
| pubmed-article:8892915 | pubmed:meshHeading | pubmed-meshheading:8892915-... | lld:pubmed |
| pubmed-article:8892915 | pubmed:meshHeading | pubmed-meshheading:8892915-... | lld:pubmed |
| pubmed-article:8892915 | pubmed:meshHeading | pubmed-meshheading:8892915-... | lld:pubmed |
| pubmed-article:8892915 | pubmed:meshHeading | pubmed-meshheading:8892915-... | lld:pubmed |
| pubmed-article:8892915 | pubmed:meshHeading | pubmed-meshheading:8892915-... | lld:pubmed |
| pubmed-article:8892915 | pubmed:meshHeading | pubmed-meshheading:8892915-... | lld:pubmed |
| pubmed-article:8892915 | pubmed:meshHeading | pubmed-meshheading:8892915-... | lld:pubmed |
| pubmed-article:8892915 | pubmed:meshHeading | pubmed-meshheading:8892915-... | lld:pubmed |
| pubmed-article:8892915 | pubmed:meshHeading | pubmed-meshheading:8892915-... | lld:pubmed |
| pubmed-article:8892915 | pubmed:meshHeading | pubmed-meshheading:8892915-... | lld:pubmed |
| pubmed-article:8892915 | pubmed:meshHeading | pubmed-meshheading:8892915-... | lld:pubmed |
| pubmed-article:8892915 | pubmed:meshHeading | pubmed-meshheading:8892915-... | lld:pubmed |
| pubmed-article:8892915 | pubmed:meshHeading | pubmed-meshheading:8892915-... | lld:pubmed |
| pubmed-article:8892915 | pubmed:meshHeading | pubmed-meshheading:8892915-... | lld:pubmed |
| pubmed-article:8892915 | pubmed:meshHeading | pubmed-meshheading:8892915-... | lld:pubmed |
| pubmed-article:8892915 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8892915 | pubmed:articleTitle | DNA immunization confers protection against murine cytomegalovirus infection. | lld:pubmed |
| pubmed-article:8892915 | pubmed:affiliation | Department of Biology, University of California, San Diego, La Jolla, 92093-0357, USA. | lld:pubmed |
