| pubmed-article:8837234 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8837234 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:8837234 | lifeskim:mentions | umls-concept:C0014792 | lld:lifeskim |
| pubmed-article:8837234 | lifeskim:mentions | umls-concept:C0596901 | lld:lifeskim |
| pubmed-article:8837234 | lifeskim:mentions | umls-concept:C1622418 | lld:lifeskim |
| pubmed-article:8837234 | lifeskim:mentions | umls-concept:C0018330 | lld:lifeskim |
| pubmed-article:8837234 | lifeskim:mentions | umls-concept:C0018338 | lld:lifeskim |
| pubmed-article:8837234 | lifeskim:mentions | umls-concept:C0699789 | lld:lifeskim |
| pubmed-article:8837234 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:8837234 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:8837234 | pubmed:dateCreated | 1996-12-11 | lld:pubmed |
| pubmed-article:8837234 | pubmed:abstractText | The present study was undertaken to characterize the export of cGMP from human erythrocytes at 37 degrees C. Inside-out membrane vesicles were exposed to cGMP and [3H]-cGMP in the presence and absence of 2 mmol l-1 ATP. In the absence of ATP, an equilibrium was reached within 15 min for the lowest tested concentration (0.65 mumol l-1), and the amount of cGMP in the vesicles was linearly correlated to the cGMP concentrations in the incubate. These observations suggest that the ATP-independent process represents passive diffusion or non-saturated binding to membrane components. In the presence of ATP, cGMP accumulated linearly during the test period (up to 120 min) and the transport into the inside-out vesicles was dependent on both low- and high-Km transport. The kinetic parameters for the low-Km process were determined after 5 and 120 min, the Km values being 4.6 (SD 1.9) and 4.7 (SD 1.1) mumol l-1 (n = 3), respectively. The corresponding Vmax values were 400 (SD 50) and 440 (SD 70) fmol mg-1 min-1. The high-Km process was characterized by Km = 170 (SD 50) mumol-1 and Vmax = 1610 (SD 280) fmol mg-1 min-1 (n = 5). The present data demonstrate an ATP-requiring saturable transport system for cGMP in human erythrocytes. | lld:pubmed |
| pubmed-article:8837234 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8837234 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8837234 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8837234 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8837234 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8837234 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8837234 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:8837234 | pubmed:issn | 0036-5513 | lld:pubmed |
| pubmed-article:8837234 | pubmed:author | pubmed-author:SagerGG | lld:pubmed |
| pubmed-article:8837234 | pubmed:author | pubmed-author:KjørstadK EKE | lld:pubmed |
| pubmed-article:8837234 | pubmed:author | pubmed-author:MoriG AGA | lld:pubmed |
| pubmed-article:8837234 | pubmed:author | pubmed-author:PettersenR... | lld:pubmed |
| pubmed-article:8837234 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8837234 | pubmed:volume | 56 | lld:pubmed |
| pubmed-article:8837234 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8837234 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8837234 | pubmed:pagination | 289-93 | lld:pubmed |
| pubmed-article:8837234 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:8837234 | pubmed:meshHeading | pubmed-meshheading:8837234-... | lld:pubmed |
| pubmed-article:8837234 | pubmed:meshHeading | pubmed-meshheading:8837234-... | lld:pubmed |
| pubmed-article:8837234 | pubmed:meshHeading | pubmed-meshheading:8837234-... | lld:pubmed |
| pubmed-article:8837234 | pubmed:meshHeading | pubmed-meshheading:8837234-... | lld:pubmed |
| pubmed-article:8837234 | pubmed:meshHeading | pubmed-meshheading:8837234-... | lld:pubmed |
| pubmed-article:8837234 | pubmed:meshHeading | pubmed-meshheading:8837234-... | lld:pubmed |
| pubmed-article:8837234 | pubmed:meshHeading | pubmed-meshheading:8837234-... | lld:pubmed |
| pubmed-article:8837234 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8837234 | pubmed:articleTitle | Export of guanosine 3',5'-cyclic monophosphate (cGMP) from human erythrocytes characterized by inside-out membrane vesicles. | lld:pubmed |
| pubmed-article:8837234 | pubmed:affiliation | Department of Pharmacology, University of Tromsø, Norway. | lld:pubmed |
| pubmed-article:8837234 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8837234 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8837234 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8837234 | lld:pubmed |
