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pubmed-article:8704944pubmed:abstractTextA rapid and selective HPLC method has been developed for the separation and quantitation of metronidazole and its hydroxylated metabolite in human plasma, saliva and gastric juice. The assay requires a simple protein precipitation step prior to analysis and is selective, sensitive and reproducible. The limits of quantitation (0.5-ml sample) were at least 0.25 microgram/ml for metronidazole and 0.20 micrograms/ml for its hydroxy metabolite. A Hypersil ODS 5 micron (150 x 4.5 mm I.D.) column was used with a mobile phase of acetonitrile-aqueous 0.05 M potassium phosphate buffer (pH 7) containing 0.1 % triethylamine (10:90) delivered at a flow-rate of 1.0 ml/min.lld:pubmed
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pubmed-article:8704944pubmed:dateRevised2007-10-16lld:pubmed
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pubmed-article:8704944pubmed:articleTitleRapid and selective high-performance liquid chromatographic method for the determination of metronidazole and its active metabolite in human plasma, saliva and gastric juice.lld:pubmed
pubmed-article:8704944pubmed:affiliationDepartment of Pharmaceutical Sciences, University of Nottingham, University Park, UK.lld:pubmed
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