| pubmed-article:8642666 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8642666 | lifeskim:mentions | umls-concept:C0022369 | lld:lifeskim |
| pubmed-article:8642666 | lifeskim:mentions | umls-concept:C0027836 | lld:lifeskim |
| pubmed-article:8642666 | lifeskim:mentions | umls-concept:C0017428 | lld:lifeskim |
| pubmed-article:8642666 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:8642666 | lifeskim:mentions | umls-concept:C0040649 | lld:lifeskim |
| pubmed-article:8642666 | lifeskim:mentions | umls-concept:C0086860 | lld:lifeskim |
| pubmed-article:8642666 | lifeskim:mentions | umls-concept:C1514562 | lld:lifeskim |
| pubmed-article:8642666 | lifeskim:mentions | umls-concept:C0205087 | lld:lifeskim |
| pubmed-article:8642666 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:8642666 | pubmed:dateCreated | 1996-7-18 | lld:pubmed |
| pubmed-article:8642666 | pubmed:abstractText | The transcription control region of the archetype strain of the human polyomavirus JC virus (JCV(Cy)), unlike its neurotropic counterpart (JCV(Mad-1)), contains only one copy of the 98-bp enhancer/promoter repeat with the 23-bp and the 66-bp insertion blocks. Early studies by us and others have indicated that the structural organization of JCV(Mad-1) is critical for glial cell-specific transcription of the viral genome. In addition, the kappa B regulatory motif found in the JCV(Mad-1) genome, which also exists in JCV(Cy), confers inducibility to the JCV(Mad-1) early and late promoters in response to extracellular stimuli. In this study, we have investigated the regulatory role of the 23- and the 66-bp blocks and their functional relationship to the kappa B motif in stimulating transcription of the Cy early and late promoters in glial cells. We demonstrate that mutations in the kappa B motif reduce the basal activity of the Cy early promoter and decrease the levels of its induction by phorbol myristate acetate or factors derived from activated T cells. Under similar circumstances, mutation in the kappa B motif completely abrogated the basal and the induced levels of transcription of the viral late promoter. Using deletion and hybrid promoter constructs, we have demonstrated that the 23-bp block of the Cy promoter plays a critical role in the observed inactivation of Cy late promoter transcription in glial cells. Results from DNA binding studies have indicated the formation of a common nucleoprotein complex with the 23-bp sequence, mutant kappa B (kappa B(mut)), and wild-type kappa B (kappa B(wt)). Analysis of this complex by UV cross-linking has identified a 40-kDa protein which binds to the 23-bp sequence and the kappa B motif. The importance of these findings for the activation of JCV(Cy) under various physiological conditions is discussed. | lld:pubmed |
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| pubmed-article:8642666 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8642666 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8642666 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:8642666 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8642666 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:8642666 | pubmed:issn | 0022-538X | lld:pubmed |
| pubmed-article:8642666 | pubmed:author | pubmed-author:RazmaraMM | lld:pubmed |
| pubmed-article:8642666 | pubmed:author | pubmed-author:KhaliliKK | lld:pubmed |
| pubmed-article:8642666 | pubmed:author | pubmed-author:SafakMM | lld:pubmed |
| pubmed-article:8642666 | pubmed:author | pubmed-author:ZoltickPP | lld:pubmed |
| pubmed-article:8642666 | pubmed:author | pubmed-author:MayreddyR PRP | lld:pubmed |
| pubmed-article:8642666 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8642666 | pubmed:volume | 70 | lld:pubmed |
| pubmed-article:8642666 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8642666 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8642666 | pubmed:pagination | 2387-93 | lld:pubmed |
| pubmed-article:8642666 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:8642666 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8642666 | pubmed:articleTitle | Transcription of the JC virus archetype late genome: importance of the kappa B and the 23-base-pair motifs in late promoter activity in glial cells. | lld:pubmed |
| pubmed-article:8642666 | pubmed:affiliation | Molecular Neurovirology, Jefferson Institute of Molecular Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA. | lld:pubmed |
| pubmed-article:8642666 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8642666 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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