| pubmed-article:8565117 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8565117 | lifeskim:mentions | umls-concept:C1003377 | lld:lifeskim |
| pubmed-article:8565117 | lifeskim:mentions | umls-concept:C0017108 | lld:lifeskim |
| pubmed-article:8565117 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:8565117 | lifeskim:mentions | umls-concept:C0020281 | lld:lifeskim |
| pubmed-article:8565117 | lifeskim:mentions | umls-concept:C0199176 | lld:lifeskim |
| pubmed-article:8565117 | lifeskim:mentions | umls-concept:C0050078 | lld:lifeskim |
| pubmed-article:8565117 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:8565117 | lifeskim:mentions | umls-concept:C2700605 | lld:lifeskim |
| pubmed-article:8565117 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:8565117 | pubmed:dateCreated | 1996-3-1 | lld:pubmed |
| pubmed-article:8565117 | pubmed:abstractText | The capability of Cr(III) to induce DNA lesions generated by oxidative damage was investigated in this study by examining the formation of 8-hydroxydeoxyguanosine (8-OHdG) in calf thymus DNA by CrCl3 and/or H2O2 in 10 mM phosphate buffer. In the presence of 0.5 mM H2O2, the formation of 8-OHdG markedly increased with increasing CrCl3 concentration. In contrast, H2O2 or CrCl3 alone did not cause any increase in 8-OHdG level above background. The amount of 8-OHdG induced by CrCl3 plus H2O2 was time dependent; its generation increased linearly over an incubation period of 90 min. The formation of 8-OHdG was unfavorable in an acidic solution (pH < 6); the highest level of 8-OHdG was observed at pH 7-8. Scavengers of reactive oxygen species markedly inhibited the formation of 8-OHdG by CrCl3 plus H2O2; the inhibition effect was sodium azide > D-mannitol > Tris-HCl at an equal concentration. The induction of 8-OHdG by CrCl3 plus H2O2 remained unchanged in D2O. Moreover, an addition of catalase (2.2 U/ml) to the reaction mixture completely inhibited the formation of 8-OHdG by CrCl3/H2O2, whereas only 22% of that formation was inhibited by superoxide dismutase (11 U/ml). A large amount of bovine serum albumin (1.1 mg/ml) could reduce the formation of 8-OHdG by CrCl3 plus H2O2, thereby implying that Cr(III)-mediated DNA-protein crosslinks are unfavorable for 8-OHdG formation. Furthermore, ascorbate could prevent the formation of 8-OHdG by CrCl3 plus H2O2; the extent of prevention increased with increasing ascorbate concentration (10 microM-3 mM). Thus, ascorbate acts as a free radical scavenger in the CrCl3/H2O2 system. The above findings suggest that Cr(III)/H2O2 could generate oxidative damage to DNA, possibly through a Fenton-like reaction, i.e. Cr(III)+H2O2-->Cr(IV)+.OH+OH-. This study also indicates that Cr(III), previously considered as the ultimate kinetically stable species of Cr(VI) metabolites, is capable of inducing carcinogenic lesions through interaction with a cellular oxygen species. | lld:pubmed |
| pubmed-article:8565117 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8565117 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8565117 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8565117 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8565117 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8565117 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8565117 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8565117 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8565117 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8565117 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8565117 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8565117 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8565117 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:8565117 | pubmed:issn | 0143-3334 | lld:pubmed |
| pubmed-article:8565117 | pubmed:author | pubmed-author:ChenC LCL | lld:pubmed |
| pubmed-article:8565117 | pubmed:author | pubmed-author:KAUQ YQY | lld:pubmed |
| pubmed-article:8565117 | pubmed:author | pubmed-author:YangJ LJL | lld:pubmed |
| pubmed-article:8565117 | pubmed:author | pubmed-author:TsouT CTC | lld:pubmed |
| pubmed-article:8565117 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8565117 | pubmed:volume | 17 | lld:pubmed |
| pubmed-article:8565117 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8565117 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8565117 | pubmed:pagination | 103-8 | lld:pubmed |
| pubmed-article:8565117 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:8565117 | pubmed:meshHeading | pubmed-meshheading:8565117-... | lld:pubmed |
| pubmed-article:8565117 | pubmed:meshHeading | pubmed-meshheading:8565117-... | lld:pubmed |
| pubmed-article:8565117 | pubmed:meshHeading | pubmed-meshheading:8565117-... | lld:pubmed |
| pubmed-article:8565117 | pubmed:meshHeading | pubmed-meshheading:8565117-... | lld:pubmed |
| pubmed-article:8565117 | pubmed:meshHeading | pubmed-meshheading:8565117-... | lld:pubmed |
| pubmed-article:8565117 | pubmed:meshHeading | pubmed-meshheading:8565117-... | lld:pubmed |
| pubmed-article:8565117 | pubmed:meshHeading | pubmed-meshheading:8565117-... | lld:pubmed |
| pubmed-article:8565117 | pubmed:meshHeading | pubmed-meshheading:8565117-... | lld:pubmed |
| pubmed-article:8565117 | pubmed:meshHeading | pubmed-meshheading:8565117-... | lld:pubmed |
| pubmed-article:8565117 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8565117 | pubmed:articleTitle | Induction of 8-hydroxydeoxyguanosine in DNA by chromium(III) plus hydrogen peroxide and its prevention by scavengers. | lld:pubmed |
| pubmed-article:8565117 | pubmed:affiliation | Department of Life Sciences, National Tsing Hua University, Taipei, Taiwan, Republic of China. | lld:pubmed |
| pubmed-article:8565117 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8565117 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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