| pubmed-article:8562059 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8562059 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:8562059 | lifeskim:mentions | umls-concept:C1135183 | lld:lifeskim |
| pubmed-article:8562059 | lifeskim:mentions | umls-concept:C0009015 | lld:lifeskim |
| pubmed-article:8562059 | lifeskim:mentions | umls-concept:C0227813 | lld:lifeskim |
| pubmed-article:8562059 | lifeskim:mentions | umls-concept:C0014533 | lld:lifeskim |
| pubmed-article:8562059 | lifeskim:mentions | umls-concept:C0051350 | lld:lifeskim |
| pubmed-article:8562059 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:8562059 | lifeskim:mentions | umls-concept:C1327616 | lld:lifeskim |
| pubmed-article:8562059 | lifeskim:mentions | umls-concept:C0006556 | lld:lifeskim |
| pubmed-article:8562059 | lifeskim:mentions | umls-concept:C0679058 | lld:lifeskim |
| pubmed-article:8562059 | lifeskim:mentions | umls-concept:C1547699 | lld:lifeskim |
| pubmed-article:8562059 | lifeskim:mentions | umls-concept:C2700640 | lld:lifeskim |
| pubmed-article:8562059 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:8562059 | pubmed:dateCreated | 1996-3-5 | lld:pubmed |
| pubmed-article:8562059 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8562059 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8562059 | pubmed:abstractText | In the preceding study (Okamura et al., 1992; Biol Reprod 47:1040-1052) we suggested that a 135-kDa protein secreted by porcine epididymis is involved in the sperm maturation. In this work, we have isolated the cDNA clone coding the 135-kDa protein in an effort to investigate its structure and function. The 135-kDa protein was purified from porcine cauda epididymal fluid. Three oligonucleotide probes were synthesized according to the amino acid sequences of N-termini of the native protein and trypsin-digested peptides. A cDNA clone hybridizing with these three probes was isolated from the cDNA library derived from the porcine proximal corpus epididymis. It encodes a novel protein with 1,006 amino acid residues in an open reading frame. Its overall amino acid sequence was significantly homologous (25.7%) to the alpha-mannosidase precursor of Dictiostelium discoideum (P34098). The 135-kDa protein could digest both p-nitro-phenyl-alpha-D-mannoside and high mannose oligo saccharide (Man8-GlcNAc2), strongly suggesting that it is an alpha-mannosidase homologue. The expression of this protein was specific to porcine and was localized to the very narrow parts of epididymis: the border of the caput and corpus epididymis. This protein may serve as a good marker for the functional differentiation in porcine epididymis. A possible role of this protein in the species-specific sperm-egg interaction is discussed. | lld:pubmed |
| pubmed-article:8562059 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8562059 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8562059 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8562059 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8562059 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8562059 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8562059 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8562059 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8562059 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:8562059 | pubmed:issn | 1040-452X | lld:pubmed |
| pubmed-article:8562059 | pubmed:author | pubmed-author:DacheuxJ LJL | lld:pubmed |
| pubmed-article:8562059 | pubmed:author | pubmed-author:SugitaYY | lld:pubmed |
| pubmed-article:8562059 | pubmed:author | pubmed-author:OkamuraNN | lld:pubmed |
| pubmed-article:8562059 | pubmed:author | pubmed-author:TambaMM | lld:pubmed |
| pubmed-article:8562059 | pubmed:author | pubmed-author:DacheuxFF | lld:pubmed |
| pubmed-article:8562059 | pubmed:author | pubmed-author:OnoeSS | lld:pubmed |
| pubmed-article:8562059 | pubmed:author | pubmed-author:MOSSD WDW | lld:pubmed |
| pubmed-article:8562059 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8562059 | pubmed:volume | 42 | lld:pubmed |
| pubmed-article:8562059 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8562059 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8562059 | pubmed:pagination | 141-8 | lld:pubmed |
| pubmed-article:8562059 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:8562059 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:8562059 | pubmed:articleTitle | Cloning of complementary DNA encoding a 135-kilodalton protein secreted from porcine corpus epididymis and its identification as an epididymis-specific alpha-mannosidase. | lld:pubmed |
| pubmed-article:8562059 | pubmed:affiliation | Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan. | lld:pubmed |
| pubmed-article:8562059 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8562059 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:396847 | entrezgene:pubmed | pubmed-article:8562059 | lld:entrezgene |
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