| pubmed-article:8468376 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8468376 | lifeskim:mentions | umls-concept:C0005889 | lld:lifeskim |
| pubmed-article:8468376 | lifeskim:mentions | umls-concept:C1521806 | lld:lifeskim |
| pubmed-article:8468376 | lifeskim:mentions | umls-concept:C0002620 | lld:lifeskim |
| pubmed-article:8468376 | lifeskim:mentions | umls-concept:C0043335 | lld:lifeskim |
| pubmed-article:8468376 | lifeskim:mentions | umls-concept:C0008565 | lld:lifeskim |
| pubmed-article:8468376 | lifeskim:mentions | umls-concept:C1524063 | lld:lifeskim |
| pubmed-article:8468376 | lifeskim:mentions | umls-concept:C0120447 | lld:lifeskim |
| pubmed-article:8468376 | lifeskim:mentions | umls-concept:C0205460 | lld:lifeskim |
| pubmed-article:8468376 | lifeskim:mentions | umls-concept:C1880156 | lld:lifeskim |
| pubmed-article:8468376 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:8468376 | pubmed:dateCreated | 1993-5-7 | lld:pubmed |
| pubmed-article:8468376 | pubmed:abstractText | A novel approach has been developed for direct injection of physiological fluids on an in-line extraction pre-column followed by column switching in order to introduce the adsorbed xenobiotic onto the analytical column. The physiological fluid is pre-treated with guanidinium solution in water (200 microliters of fluid plus 300 microliters of a reagent containing 8.05 M guanidinium and 1.02 M ammonium sulfate) in order to denature protein binding sites and to serve as a universal solvent for a divergent range of polar to non-polar xenobiotics in a hydrophilic medium. A 0.5 M ammonium sulfate solution (500 microliters) is used as a pre- and post-flush reagent for the extraction pre-column (30 mm x 2.1 mm I.D.). The pre-flush reagent prepares the sorbent environment of the C18 pre-column for the hydrophobic retention of analytes. The post-flush reagent flushes non-retained sample proteins and salts to waste prior to switching the pre-column in-line with the analytical column. Universal chromatographic conditions for the analytical phase allows elution of a range of polar to non-polar xenobiotics within 20 min from an end-capped C8 silica analytical column (250 mm x 4.6 mm I.D.). This is effected by a linear gradient from a binary system consisting of solvent A (0.05 M KH2PO4) and solvent B (acetonitrile-isopropanol, 80:20, v/v). | lld:pubmed |
| pubmed-article:8468376 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8468376 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8468376 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8468376 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8468376 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8468376 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8468376 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8468376 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8468376 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8468376 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8468376 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8468376 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:8468376 | pubmed:issn | 0021-9673 | lld:pubmed |
| pubmed-article:8468376 | pubmed:author | pubmed-author:AlbrechtC FCF | lld:pubmed |
| pubmed-article:8468376 | pubmed:author | pubmed-author:KrugerP BPB | lld:pubmed |
| pubmed-article:8468376 | pubmed:author | pubmed-author:Van... | lld:pubmed |
| pubmed-article:8468376 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8468376 | pubmed:day | 26 | lld:pubmed |
| pubmed-article:8468376 | pubmed:volume | 612 | lld:pubmed |
| pubmed-article:8468376 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8468376 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8468376 | pubmed:pagination | 191-8 | lld:pubmed |
| pubmed-article:8468376 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
| pubmed-article:8468376 | pubmed:meshHeading | pubmed-meshheading:8468376-... | lld:pubmed |
| pubmed-article:8468376 | pubmed:meshHeading | pubmed-meshheading:8468376-... | lld:pubmed |
| pubmed-article:8468376 | pubmed:meshHeading | pubmed-meshheading:8468376-... | lld:pubmed |
| pubmed-article:8468376 | pubmed:meshHeading | pubmed-meshheading:8468376-... | lld:pubmed |
| pubmed-article:8468376 | pubmed:meshHeading | pubmed-meshheading:8468376-... | lld:pubmed |
| pubmed-article:8468376 | pubmed:meshHeading | pubmed-meshheading:8468376-... | lld:pubmed |
| pubmed-article:8468376 | pubmed:meshHeading | pubmed-meshheading:8468376-... | lld:pubmed |
| pubmed-article:8468376 | pubmed:meshHeading | pubmed-meshheading:8468376-... | lld:pubmed |
| pubmed-article:8468376 | pubmed:meshHeading | pubmed-meshheading:8468376-... | lld:pubmed |
| pubmed-article:8468376 | pubmed:meshHeading | pubmed-meshheading:8468376-... | lld:pubmed |
| pubmed-article:8468376 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8468376 | pubmed:articleTitle | Use of guanidine hydrochloride and ammonium sulfate in comprehensive in-line sorption enrichment of xenobiotics in biological fluids by high-performance liquid chromatography. | lld:pubmed |
| pubmed-article:8468376 | pubmed:affiliation | Department of Pharmacology, Faculty of Medicine, University of Stellenbosch, Tygerberg, South Africa. | lld:pubmed |
| pubmed-article:8468376 | pubmed:publicationType | Journal Article | lld:pubmed |
