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pubmed-article:8407896pubmed:abstractTextPrevious attempts to express glycosylphosphatidylinositol-anchored proteins in Ltk- cells have not been successful because Ltk- cells cannot synthesize N-acetylglucosamine-phosphatidylinositol, the first intermediate in anchor biosynthesis. Using complementation cloning, we have identified a human cDNA that corrects the defect in anchor biosynthesis and allows the expression of glycosylphosphatidylinositol-anchored proteins in Ltk- cells. The nucleotide sequence predicts a novel cytosolic protein of 188 amino acids.lld:pubmed
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pubmed-article:8407896pubmed:articleTitleCorrection of the class H defect in glycosylphosphatidylinositol anchor biosynthesis in Ltk- cells by a human cDNA clone.lld:pubmed
pubmed-article:8407896pubmed:affiliationDepartment of Medicine, University of Texas Health Science Center at Houston 77030.lld:pubmed
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