| pubmed-article:8374038 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8374038 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:8374038 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:8374038 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:8374038 | pubmed:dateCreated | 1993-10-18 | lld:pubmed |
| pubmed-article:8374038 | pubmed:abstractText | An immunochemical assay was developed to detect carbonyl moieties that result from oxidative damage to proteins. Bovine serum albumin was reacted with hydroxyl radicals generated via a Fenton-like mechanism or by a radiolysis mechanism. The resulting albumin-derived carbonyls were reacted with 2,4-dinitrophenylhydrazine, giving the corresponding hydrazones, which were detected by Western blot using anti-dinitrophenyl antisera. The immunoblot demonstrated a concentration-dependent increase in carbonyl formation, as well as fragmentation of the albumin into two distinct bands with molecular masses of 51 and 45 kDa when oxidized with the Fenton-like mechanism, and 62 and 46 kDa when oxidized by radiolysis. Analysis of the immunoblot using laser densitometry indicated a linear relationship between carbonyl groups and increasing treatment from radiolysis. This immunochemical assay was approximately 3 orders of magnitude more sensitive than the spectrophotometric method and was able to determine the molecular mass of carbonyl-modified polypeptides in the detection of oxidative damage. | lld:pubmed |
| pubmed-article:8374038 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8374038 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:8374038 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:8374038 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8374038 | pubmed:issn | 0893-228X | lld:pubmed |
| pubmed-article:8374038 | pubmed:author | pubmed-author:HinsonJ AJA | lld:pubmed |
| pubmed-article:8374038 | pubmed:author | pubmed-author:KellerR JRJ | lld:pubmed |
| pubmed-article:8374038 | pubmed:author | pubmed-author:PumfordN RNR | lld:pubmed |
| pubmed-article:8374038 | pubmed:author | pubmed-author:HalmesN CNC | lld:pubmed |
| pubmed-article:8374038 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8374038 | pubmed:volume | 6 | lld:pubmed |
| pubmed-article:8374038 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8374038 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8374038 | pubmed:pagination | 430-3 | lld:pubmed |
| pubmed-article:8374038 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:8374038 | pubmed:articleTitle | Immunochemical detection of oxidized proteins. | lld:pubmed |
| pubmed-article:8374038 | pubmed:affiliation | Occupational and Environmental Health Program, University of Arkansas for Medical Sciences, Little Rock 72205-7199. | lld:pubmed |
| pubmed-article:8374038 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8374038 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:8374038 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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