pubmed-article:8344411 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:8344411 | lifeskim:mentions | umls-concept:C0007452 | lld:lifeskim |
pubmed-article:8344411 | lifeskim:mentions | umls-concept:C0018894 | lld:lifeskim |
pubmed-article:8344411 | lifeskim:mentions | umls-concept:C0003339 | lld:lifeskim |
pubmed-article:8344411 | lifeskim:mentions | umls-concept:C0169904 | lld:lifeskim |
pubmed-article:8344411 | lifeskim:mentions | umls-concept:C1704632 | lld:lifeskim |
pubmed-article:8344411 | lifeskim:mentions | umls-concept:C0871261 | lld:lifeskim |
pubmed-article:8344411 | lifeskim:mentions | umls-concept:C2911692 | lld:lifeskim |
pubmed-article:8344411 | lifeskim:mentions | umls-concept:C1706817 | lld:lifeskim |
pubmed-article:8344411 | lifeskim:mentions | umls-concept:C0004573 | lld:lifeskim |
pubmed-article:8344411 | lifeskim:mentions | umls-concept:C0444659 | lld:lifeskim |
pubmed-article:8344411 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
pubmed-article:8344411 | pubmed:issue | 1 | lld:pubmed |
pubmed-article:8344411 | pubmed:dateCreated | 1993-9-7 | lld:pubmed |
pubmed-article:8344411 | pubmed:abstractText | The Babesia bovis major merozoite surface antigen (MSA-1) is a 42-kDa integral membrane glycoprotein previously shown to induce immunodominant antibody responses in cattle protectively immune to B. bovis and to induce neutralizing antibody. Recent studies have also shown that MSA-1 B cell epitopes common to New World strains of B. bovis are not present in either Israel or Australia strains. To understand the potential role of this protein in protective immunity, T helper cell responses specific for MSA-1 were characterized in Babesia-immune cattle. Peripheral blood mononuclear cells from immune cattle proliferated against affinity-purified recombinant MSA-1 protein expressed in Escherichia coli. MSA-1 preferentially stimulated the growth of CD4+ T cells in cell lines cultured with antigen for 4 weeks. MSA-1-reactive cell lines responded to a membrane fraction of B. bovis merozoites, suggesting recognition of the native protein. However, B. bovis-reactive T cell lines and T helper clones established by stimulation with crude parasite membrane antigen failed to respond to recombinant MSA-1, indicating that this antigen is not immunodominant for T cells. The majority of MSA-1-specific T helper clones reacted to unfractionated merozoite membrane antigen from New World B. bovis strains, but none of the clones responded to Australia B. bovis or to a Mexico strain of Babesia bigemina. Several T helper clones produced low levels of cytokines when stimulated with concanavalin A and interleukin-2. Northern blot analysis revealed the expression of interleukin-2, interleukin-4, interferon-gamma, and tumor necrosis factor-alpha messenger RNA in mitogen-stimulated T helper clones, showing that the clones examined expressed an unrestricted T helper phenotype. We conclude that the MSA-1 protein, although serologically immunodominant and capable of inducing neutralizing antibodies as well as a T helper cell response, is not an immunodominant T cell antigen. Furthermore, the parasite strain specificity of the Th clones supports previous findings of extensive polymorphism in the MSA-1 glycoprotein and suggests that like B cell epitopes, T cell epitopes reside in a nonconserved portion of the protein. | lld:pubmed |
pubmed-article:8344411 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8344411 | pubmed:language | eng | lld:pubmed |
pubmed-article:8344411 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8344411 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:8344411 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8344411 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8344411 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8344411 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8344411 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8344411 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8344411 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8344411 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:8344411 | pubmed:month | Aug | lld:pubmed |
pubmed-article:8344411 | pubmed:issn | 0014-4894 | lld:pubmed |
pubmed-article:8344411 | pubmed:author | pubmed-author:BrownW CWC | lld:pubmed |
pubmed-article:8344411 | pubmed:author | pubmed-author:PalmerG HGH | lld:pubmed |
pubmed-article:8344411 | pubmed:author | pubmed-author:HinesS ASA | lld:pubmed |
pubmed-article:8344411 | pubmed:author | pubmed-author:McElwainT FTF | lld:pubmed |
pubmed-article:8344411 | pubmed:author | pubmed-author:DobbelaereD... | lld:pubmed |
pubmed-article:8344411 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:8344411 | pubmed:volume | 77 | lld:pubmed |
pubmed-article:8344411 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:8344411 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:8344411 | pubmed:pagination | 97-110 | lld:pubmed |
pubmed-article:8344411 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
pubmed-article:8344411 | pubmed:meshHeading | pubmed-meshheading:8344411-... | lld:pubmed |
pubmed-article:8344411 | pubmed:meshHeading | pubmed-meshheading:8344411-... | lld:pubmed |
pubmed-article:8344411 | pubmed:meshHeading | pubmed-meshheading:8344411-... | lld:pubmed |
pubmed-article:8344411 | pubmed:meshHeading | pubmed-meshheading:8344411-... | lld:pubmed |
pubmed-article:8344411 | pubmed:meshHeading | pubmed-meshheading:8344411-... | lld:pubmed |
pubmed-article:8344411 | pubmed:meshHeading | pubmed-meshheading:8344411-... | lld:pubmed |
pubmed-article:8344411 | pubmed:meshHeading | pubmed-meshheading:8344411-... | lld:pubmed |
pubmed-article:8344411 | pubmed:meshHeading | pubmed-meshheading:8344411-... | lld:pubmed |
pubmed-article:8344411 | pubmed:meshHeading | pubmed-meshheading:8344411-... | lld:pubmed |
pubmed-article:8344411 | pubmed:meshHeading | pubmed-meshheading:8344411-... | lld:pubmed |
pubmed-article:8344411 | pubmed:meshHeading | pubmed-meshheading:8344411-... | lld:pubmed |
pubmed-article:8344411 | pubmed:meshHeading | pubmed-meshheading:8344411-... | lld:pubmed |
pubmed-article:8344411 | pubmed:meshHeading | pubmed-meshheading:8344411-... | lld:pubmed |
pubmed-article:8344411 | pubmed:meshHeading | pubmed-meshheading:8344411-... | lld:pubmed |
pubmed-article:8344411 | pubmed:meshHeading | pubmed-meshheading:8344411-... | lld:pubmed |
pubmed-article:8344411 | pubmed:meshHeading | pubmed-meshheading:8344411-... | lld:pubmed |
pubmed-article:8344411 | pubmed:meshHeading | pubmed-meshheading:8344411-... | lld:pubmed |
pubmed-article:8344411 | pubmed:year | 1993 | lld:pubmed |
pubmed-article:8344411 | pubmed:articleTitle | Babesia bovis: characterization of the T helper cell response against the 42-kDa merozoite surface antigen (MSA-1) in cattle. | lld:pubmed |
pubmed-article:8344411 | pubmed:affiliation | Department of Veterinary Pathobiology, Texas A&M University, College Station 77843. | lld:pubmed |
pubmed-article:8344411 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:8344411 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
pubmed-article:8344411 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8344411 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8344411 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8344411 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8344411 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8344411 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8344411 | lld:pubmed |