| pubmed-article:8342602 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8342602 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:8342602 | lifeskim:mentions | umls-concept:C0020202 | lld:lifeskim |
| pubmed-article:8342602 | lifeskim:mentions | umls-concept:C0001418 | lld:lifeskim |
| pubmed-article:8342602 | lifeskim:mentions | umls-concept:C0007097 | lld:lifeskim |
| pubmed-article:8342602 | lifeskim:mentions | umls-concept:C0030274 | lld:lifeskim |
| pubmed-article:8342602 | lifeskim:mentions | umls-concept:C0028953 | lld:lifeskim |
| pubmed-article:8342602 | lifeskim:mentions | umls-concept:C0032520 | lld:lifeskim |
| pubmed-article:8342602 | lifeskim:mentions | umls-concept:C1518575 | lld:lifeskim |
| pubmed-article:8342602 | lifeskim:mentions | umls-concept:C0022457 | lld:lifeskim |
| pubmed-article:8342602 | lifeskim:mentions | umls-concept:C2603343 | lld:lifeskim |
| pubmed-article:8342602 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:8342602 | lifeskim:mentions | umls-concept:C0205195 | lld:lifeskim |
| pubmed-article:8342602 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:8342602 | pubmed:dateCreated | 1993-9-2 | lld:pubmed |
| pubmed-article:8342602 | pubmed:abstractText | We examined 82 surgically resected or biopsied, formalin-fixed, paraffin-embedded primary adenocarcinomas of the pancreas for the presence of activating point mutations in codon 12 of the K-ras oncogene. Mutations were detected using primer-mediated, mutant-enriched, polymerase chain reaction-restriction fragment length polymorphism analysis and characterized further by allele-specific oligonucleotide hybridization. This combination of mutant-enriched polymerase chain reaction-restriction fragment length polymorphism analysis and allele-specific oligonucleotide hybridization results in a rapid and sensitive characterization of the mutations in codon 12 of K-ras. Sixty-eight (83%) of the 82 carcinomas examined harbored a point mutation. Of the 68 mutations, 33 (49%) were guanine to adenine transitions, 27 (39%) were guanine to thymine transversions, and eight (12%) were guanine to cytosine transversions. Mutations were found in carcinomas of the head (61 of 75, 81%) as well as in carcinomas of the body or tail (seven of seven, 100%) of the pancreas. The overall prevalence of K-ras point mutations in adenocarcinomas of the pancreas obtained from patients who smoked cigarettes at some point during their lives (88%; 86% in current smokers and 89% in ex-smokers) was greater than that seen in pancreatic adenocarcinomas from patients who never smoked cigarettes (68%, P = 0.046). The presence of K-ras point mutations did not correlate with tumor ploidy, tumor proliferating index, or patient survival. These results demonstrate that primer-mediated, mutant-enriched polymerase chain reaction-restriction fragment length polymorphism analysis combined with allele-specific oligonucleotide hybridization can be used to detect and characterize mutations in codon 12 of the K-ras oncogene in formalin-fixed, paraffin-embedded tissues, and the results confirm that activating point mutations in codon 12 of the K-ras oncogene occur frequently in adenocarcinomas of the pancreas. | lld:pubmed |
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| pubmed-article:8342602 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8342602 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8342602 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:8342602 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8342602 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8342602 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8342602 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:8342602 | pubmed:issn | 0002-9440 | lld:pubmed |
| pubmed-article:8342602 | pubmed:author | pubmed-author:KenslerT WTW | lld:pubmed |
| pubmed-article:8342602 | pubmed:author | pubmed-author:CameronJ LJL | lld:pubmed |
| pubmed-article:8342602 | pubmed:author | pubmed-author:BosJ LJL | lld:pubmed |
| pubmed-article:8342602 | pubmed:author | pubmed-author:van... | lld:pubmed |
| pubmed-article:8342602 | pubmed:author | pubmed-author:BoseK KKK | lld:pubmed |
| pubmed-article:8342602 | pubmed:author | pubmed-author:HrubanR HRH | lld:pubmed |
| pubmed-article:8342602 | pubmed:author | pubmed-author:OfferhausG... | lld:pubmed |
| pubmed-article:8342602 | pubmed:author | pubmed-author:GoodmanS NSN | lld:pubmed |
| pubmed-article:8342602 | pubmed:author | pubmed-author:AllisonD CDC | lld:pubmed |
| pubmed-article:8342602 | pubmed:author | pubmed-author:van... | lld:pubmed |
| pubmed-article:8342602 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8342602 | pubmed:volume | 143 | lld:pubmed |
| pubmed-article:8342602 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8342602 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8342602 | pubmed:pagination | 545-54 | lld:pubmed |
| pubmed-article:8342602 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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