| pubmed-article:8335946 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8335946 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:8335946 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:8335946 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:8335946 | lifeskim:mentions | umls-concept:C0024432 | lld:lifeskim |
| pubmed-article:8335946 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:8335946 | lifeskim:mentions | umls-concept:C1327616 | lld:lifeskim |
| pubmed-article:8335946 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:8335946 | pubmed:dateCreated | 1993-8-26 | lld:pubmed |
| pubmed-article:8335946 | pubmed:abstractText | The conditioned medium of the murine macrophage PU5.1.8 was analyzed by two-dimensional gel electrophoresis in order to detect LPS-induced proteins. Spots of interest were identified by microsequencing of internal peptides generated by limited in situ acid hydrolysis. In total conditioned medium, several monokines (TNF-alpha and macrophage inflammatory protein-1 alpha and 1 beta) were identified as LPS-induced spots. Because minor spots could be masked by the complexity of the 2-D pattern, conditioned medium was successively fractionated by zinc precipitation and affinity chromatography (Procion red and Con A agarose). Zinc supernatant fraction, Procion red flow-through, and Con A eluate fractions were further analyzed by 2-D gel electrophoresis for the presence of LPS-induced spots. In these fractions serum amyloid A3, lipocalin 24p3, cathepsin B, and plasminogen activator inhibitor-I were characterized as LPS-induced proteins secreted by macrophages. Lipocalin 24p3 protein was retrieved for the first time. In addition to these proteins that follow a classical secretory pathway, several cellular proteins (mainly ribosomal proteins) were retrieved as LPS-induced proteins in the conditioned medium. Control experiments argue against the obvious explanation that the latter observation is caused solely by cellular leakage. | lld:pubmed |
| pubmed-article:8335946 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8335946 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8335946 | pubmed:citationSubset | AIM | lld:pubmed |
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| pubmed-article:8335946 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8335946 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:8335946 | pubmed:issn | 0022-1767 | lld:pubmed |
| pubmed-article:8335946 | pubmed:author | pubmed-author:Van de... | lld:pubmed |
| pubmed-article:8335946 | pubmed:author | pubmed-author:Van... | lld:pubmed |
| pubmed-article:8335946 | pubmed:author | pubmed-author:Van BunS MSM | lld:pubmed |
| pubmed-article:8335946 | pubmed:author | pubmed-author:MeheusL ALA | lld:pubmed |
| pubmed-article:8335946 | pubmed:author | pubmed-author:RaymackersJ... | lld:pubmed |
| pubmed-article:8335946 | pubmed:author | pubmed-author:FransenL MLM | lld:pubmed |
| pubmed-article:8335946 | pubmed:author | pubmed-author:BlockxH AHA | lld:pubmed |
| pubmed-article:8335946 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8335946 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:8335946 | pubmed:volume | 151 | lld:pubmed |
| pubmed-article:8335946 | pubmed:geneSymbol | SAA3 | lld:pubmed |
| pubmed-article:8335946 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8335946 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8335946 | pubmed:pagination | 1535-47 | lld:pubmed |
| pubmed-article:8335946 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:8335946 | pubmed:meshHeading | pubmed-meshheading:8335946-... | lld:pubmed |
| pubmed-article:8335946 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8335946 | pubmed:articleTitle | Identification by microsequencing of lipopolysaccharide-induced proteins secreted by mouse macrophages. | lld:pubmed |
| pubmed-article:8335946 | pubmed:affiliation | Innogenetics N.V., Industripark Zwijnaarde, Belgium. | lld:pubmed |
| pubmed-article:8335946 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8335946 | pubmed:publicationType | In Vitro | lld:pubmed |
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