| pubmed-article:8331595 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8331595 | lifeskim:mentions | umls-concept:C0027882 | lld:lifeskim |
| pubmed-article:8331595 | lifeskim:mentions | umls-concept:C0927232 | lld:lifeskim |
| pubmed-article:8331595 | lifeskim:mentions | umls-concept:C2004454 | lld:lifeskim |
| pubmed-article:8331595 | lifeskim:mentions | umls-concept:C0023209 | lld:lifeskim |
| pubmed-article:8331595 | lifeskim:mentions | umls-concept:C1655751 | lld:lifeskim |
| pubmed-article:8331595 | pubmed:dateCreated | 1993-8-18 | lld:pubmed |
| pubmed-article:8331595 | pubmed:abstractText | 1. Neutral-carrier pH-sensitive microelectrodes were used to investigate intracellular pH (pHi) recovery from alkalinization in leech Retzius neurones in Hepes- and in CO2-HCO3(-)-buffered solution. The Retzius neurones were alkaline loaded by the addition and subsequent removal of 16 mM acetate, by changing from 5% CO2-27 mM HCO3- to 2% CO2-11 mM HCO3- or by changing from CO2-HCO3(-)- to Hepes-buffered solution. 2. In Hepes-buffered solution (pH 7.4) the mean pHi was 7.29 +/- 0.11 and the mean membrane potential -44.7 +/- 5.9 mV (mean +/- S.D.; n = 83). 3. The rate of pHi recovery from alkalinization increased with decreasing pH of the bathing medium (pHb). pHi changed about 0.30 pH units for a pHb unit change. 4. A decrease of extracellular buffer concentration (Hepes concentration lowered from 20 to 5 mM) caused an acidification of extracellular and intracellular pH and an acceleration of pHi recovery from alkalinization. 5. A depolarization of the Retzius cell membrane-induced by increasing the K+ concentration of the bathing medium from 4 to 20 mM (delta Em = 16.5 +/- 5.5 mV) or from 4 to 40 mM (delta Em = 24.8 +/- 3.5 mV)--evoked a decrease of pHi and an acceleration of pHi recovery from alkalinization. 6. The H+ current blocker Zn2+ (0.5 mM) inhibited pHi recovery from alkalinization at resting membrane potential as well as during depolarization. The inhibition was more pronounced during depolarization. 7. In Cl(-)-free, CO2-HCO3(-)-buffered solution pHi recovery from an alkaline load by changing from 5% CO2-27 mM HCO3- to 2% CO2-11 mM HCO3- was slowed by 48-71%. The rate of pHi recovery from an alkaline load induced by changing from CO2-HCO3- to Hepes buffer was reduced by 33-56% in Cl(-)-free solution. The removal of external Cl- did not affect pHi recovery in Hepes-buffered solution. 8. The pHi recovery from alkalinization was DIDS-insensitive in CO2-HCO3(-)- as in Hepes-buffered solutions and was not slowed in the absence of external Na+. 9. It is concluded that in Retzius neurones pHi recovery from alkalinization is mediated by a passive voltage-dependent H+ influx along the electrochemical proton gradient. In the presence of CO2-HCO3- buffer a DIDS-insensitive Cl(-)-HCO3- exchanger additionally regulates pHi after an intracellular alkaline load. It cannot be excluded that intracellular processes (e.g. H+ release from organelles, metabolic H+ production) are also involved in pHi recovery from alkalinization. | lld:pubmed |
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| pubmed-article:8331595 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8331595 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8331595 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:8331595 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8331595 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:8331595 | pubmed:issn | 0022-3751 | lld:pubmed |
| pubmed-article:8331595 | pubmed:author | pubmed-author:FreyGG | lld:pubmed |
| pubmed-article:8331595 | pubmed:author | pubmed-author:SchlueW RWR | lld:pubmed |
| pubmed-article:8331595 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8331595 | pubmed:volume | 462 | lld:pubmed |
| pubmed-article:8331595 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8331595 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8331595 | pubmed:pagination | 627-43 | lld:pubmed |
| pubmed-article:8331595 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:8331595 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8331595 | pubmed:articleTitle | pH recovery from intracellular alkalinization in Retzius neurones of the leech central nervous system. | lld:pubmed |
| pubmed-article:8331595 | pubmed:affiliation | Institut für Zoologie/Lehrstuhl für Neurobiologie, Heinrich-Heine-Universität, Düsseldorf, Germany. | lld:pubmed |
| pubmed-article:8331595 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8331595 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8331595 | lld:pubmed |
