| pubmed-article:8243889 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8243889 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:8243889 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:8243889 | lifeskim:mentions | umls-concept:C0206664 | lld:lifeskim |
| pubmed-article:8243889 | lifeskim:mentions | umls-concept:C0040845 | lld:lifeskim |
| pubmed-article:8243889 | lifeskim:mentions | umls-concept:C0031437 | lld:lifeskim |
| pubmed-article:8243889 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:8243889 | lifeskim:mentions | umls-concept:C2348205 | lld:lifeskim |
| pubmed-article:8243889 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:8243889 | pubmed:dateCreated | 1993-12-23 | lld:pubmed |
| pubmed-article:8243889 | pubmed:abstractText | Prior work has shown that all-trans retinoic acid (t-RA) treatment of the human teratocarcinoma (TC) cell line NTERA-2 clone D1 (abbreviated NT2/D1) induces a neuronal phenotype and other cell lineages. This study sought to explore the potential of 9-cis retinoic acid (9-cis RA) as a differentiation-inducing agent of this multipotent cell. Findings reported here show that 9-cis RA induced a phenotype similar to t-RA treatment of NT2/D1 cells. This similarity extended to their effects on the nuclear receptors retinoic acid receptor-beta (RAR-beta) and retinoid X receptor-alpha (RXR-alpha). Both retinoids prominently augmented RAR-beta expression and transactivated a reporter plasmid containing putative RAR response elements (RAREs) with direct repeats separated by five nucleotides (DR5). Both retinoids had no appreciable effect on RXR-alpha expression and both minimally transactivated a reporter plasmid containing putative RXR response elements (RXREs) with direct repeats separated by one nucleotide (DR1). These studies suggest that 9-cis RA and t-RA activate common events during retinoid-mediated NT2/D1 differentiation. This hypothesis was supported by the finding that NT2/D1 cells rendered refractory to t-RA (NT2/D1-R1) were also resistant to 9-cis RA. To discover alterations that could confer retinoid-refractoriness, retinoid receptor expression was examined in NT2/D1-R1 cells. In contrast to NT2/D1, the NT2/D1-R1 cell was found to have reduced RXR-alpha expression at the level of total cellular RNA.(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
| pubmed-article:8243889 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8243889 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8243889 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8243889 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8243889 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8243889 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8243889 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8243889 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8243889 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:8243889 | pubmed:issn | 0301-4681 | lld:pubmed |
| pubmed-article:8243889 | pubmed:author | pubmed-author:BuckJJ | lld:pubmed |
| pubmed-article:8243889 | pubmed:author | pubmed-author:MorCC | lld:pubmed |
| pubmed-article:8243889 | pubmed:author | pubmed-author:DmitrovskyEE | lld:pubmed |
| pubmed-article:8243889 | pubmed:author | pubmed-author:KurieJ MJM | lld:pubmed |
| pubmed-article:8243889 | pubmed:author | pubmed-author:EppingerT MTM | lld:pubmed |
| pubmed-article:8243889 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8243889 | pubmed:volume | 54 | lld:pubmed |
| pubmed-article:8243889 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8243889 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8243889 | pubmed:pagination | 123-9 | lld:pubmed |
| pubmed-article:8243889 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:8243889 | pubmed:meshHeading | pubmed-meshheading:8243889-... | lld:pubmed |
| pubmed-article:8243889 | pubmed:meshHeading | pubmed-meshheading:8243889-... | lld:pubmed |
| pubmed-article:8243889 | pubmed:meshHeading | pubmed-meshheading:8243889-... | lld:pubmed |
| pubmed-article:8243889 | pubmed:meshHeading | pubmed-meshheading:8243889-... | lld:pubmed |
| pubmed-article:8243889 | pubmed:meshHeading | pubmed-meshheading:8243889-... | lld:pubmed |
| pubmed-article:8243889 | pubmed:meshHeading | pubmed-meshheading:8243889-... | lld:pubmed |
| pubmed-article:8243889 | pubmed:meshHeading | pubmed-meshheading:8243889-... | lld:pubmed |
| pubmed-article:8243889 | pubmed:meshHeading | pubmed-meshheading:8243889-... | lld:pubmed |
| pubmed-article:8243889 | pubmed:meshHeading | pubmed-meshheading:8243889-... | lld:pubmed |
| pubmed-article:8243889 | pubmed:meshHeading | pubmed-meshheading:8243889-... | lld:pubmed |
| pubmed-article:8243889 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8243889 | pubmed:articleTitle | 9-cis and all-trans retinoic acid induce a similar phenotype in human teratocarcinoma cells. | lld:pubmed |
| pubmed-article:8243889 | pubmed:affiliation | Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York, NY 10021. | lld:pubmed |
| pubmed-article:8243889 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8243889 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:8243889 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8243889 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
