pubmed-article:8221657 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:8221657 | lifeskim:mentions | umls-concept:C0149925 | lld:lifeskim |
pubmed-article:8221657 | lifeskim:mentions | umls-concept:C1704387 | lld:lifeskim |
pubmed-article:8221657 | lifeskim:mentions | umls-concept:C1522642 | lld:lifeskim |
pubmed-article:8221657 | lifeskim:mentions | umls-concept:C0597357 | lld:lifeskim |
pubmed-article:8221657 | lifeskim:mentions | umls-concept:C1171362 | lld:lifeskim |
pubmed-article:8221657 | lifeskim:mentions | umls-concept:C0300926 | lld:lifeskim |
pubmed-article:8221657 | lifeskim:mentions | umls-concept:C0018270 | lld:lifeskim |
pubmed-article:8221657 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
pubmed-article:8221657 | lifeskim:mentions | umls-concept:C1515670 | lld:lifeskim |
pubmed-article:8221657 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
pubmed-article:8221657 | lifeskim:mentions | umls-concept:C0086597 | lld:lifeskim |
pubmed-article:8221657 | lifeskim:mentions | umls-concept:C1553412 | lld:lifeskim |
pubmed-article:8221657 | lifeskim:mentions | umls-concept:C1548328 | lld:lifeskim |
pubmed-article:8221657 | pubmed:issue | 21 | lld:pubmed |
pubmed-article:8221657 | pubmed:dateCreated | 1993-11-29 | lld:pubmed |
pubmed-article:8221657 | pubmed:abstractText | Gastrin, cholecystokinin (CCK), and CCK-related peptides comprise a hormonal family characterized by an identical carboxy-terminal amino acid sequence, a domain critical for receptor binding. The addition of gastrin to small cell lung cancer (SCLC) cells causes a rapid and transient increase in the intracellular concentration of calcium ([Ca2+]i). Furthermore, gastrin acts as a direct growth factor through CCKB/gastrin receptors. We report here that the expression of the mRNA coding for CCKB/gastrin receptors correlates with the responsiveness of SCLC cells to gastrin in terms of Ca2+ mobilization and stimulation of clonal growth in semisolid medium. The GLC19 SCLC cell line had no detectable expression of CCKB/gastrin receptor mRNA. Accordingly, gastrin (1-100 nM) did not cause any measurable increase in [Ca2+]i. In contrast, the addition of cholecystokinin residues 26-33 (CCK-8) caused a rapid and transient increase in [Ca2+]i in this cell line. CCK-8 mobilized Ca2+ in a dose-dependent manner in the nanomolar range (half-maximal stimulatory concentration = 12 nM). Furthermore, the selective CCKA antagonist CAM-1481 inhibited the increase in [Ca2+]i induced by CCK-8 (half-maximal inhibitory concentration = 3 nM) in GLC19 but not in H510 cells. The selective CCKB/gastrin antagonist blocked the increase in [Ca2+]i induced by CCK-8 (half-maximal inhibitory concentration = 80 pM) in H510 but not in GLC19 cells. Thus, the effects of CCK-8 are mediated through CCKA receptors in GLC19 cells and via CCKB/gastrin receptors in H510 cells. CCK-8 markedly stimulated colony formation in GLC19 cells in a dose-dependent manner in the nanomolar range, whereas over the same concentration range, gastrin had no effect on clonal growth. CAM-1481 inhibited the CCK-stimulated colony formation in GLC19 but not in H510 cells. Our results show, for the first time, that CCKA receptors can mediate Ca2+ mobilization and growth in SCLC cells and that SCLC cells express two distinct functional CCK receptor subtypes. | lld:pubmed |
pubmed-article:8221657 | pubmed:language | eng | lld:pubmed |
pubmed-article:8221657 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8221657 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:8221657 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8221657 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8221657 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8221657 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8221657 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8221657 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8221657 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8221657 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8221657 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:8221657 | pubmed:month | Nov | lld:pubmed |
pubmed-article:8221657 | pubmed:issn | 0008-5472 | lld:pubmed |
pubmed-article:8221657 | pubmed:author | pubmed-author:WalshJ HJH | lld:pubmed |
pubmed-article:8221657 | pubmed:author | pubmed-author:RozengurtEE | lld:pubmed |
pubmed-article:8221657 | pubmed:author | pubmed-author:NeuII | lld:pubmed |
pubmed-article:8221657 | pubmed:author | pubmed-author:SethiTT | lld:pubmed |
pubmed-article:8221657 | pubmed:author | pubmed-author:HergetTT | lld:pubmed |
pubmed-article:8221657 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:8221657 | pubmed:day | 1 | lld:pubmed |
pubmed-article:8221657 | pubmed:volume | 53 | lld:pubmed |
pubmed-article:8221657 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:8221657 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:8221657 | pubmed:pagination | 5208-13 | lld:pubmed |
pubmed-article:8221657 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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pubmed-article:8221657 | pubmed:year | 1993 | lld:pubmed |
pubmed-article:8221657 | pubmed:articleTitle | CCKA and CCKB receptors are expressed in small cell lung cancer lines and mediate Ca2+ mobilization and clonal growth. | lld:pubmed |
pubmed-article:8221657 | pubmed:affiliation | Imperial Cancer Research Fund, London, England. | lld:pubmed |
pubmed-article:8221657 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:8221657 | pubmed:publicationType | Comparative Study | lld:pubmed |
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