| pubmed-article:8167472 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8167472 | lifeskim:mentions | umls-concept:C0017387 | lld:lifeskim |
| pubmed-article:8167472 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:8167472 | lifeskim:mentions | umls-concept:C0521119 | lld:lifeskim |
| pubmed-article:8167472 | lifeskim:mentions | umls-concept:C0184661 | lld:lifeskim |
| pubmed-article:8167472 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:8167472 | lifeskim:mentions | umls-concept:C0163344 | lld:lifeskim |
| pubmed-article:8167472 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:8167472 | pubmed:dateCreated | 1994-5-27 | lld:pubmed |
| pubmed-article:8167472 | pubmed:abstractText | Overproduction of the extracellular Serratia marcescens nuclease in Escherichia coli results in aggregation and sequestration of a large amount of the protein in inclusion bodies. Only a relatively small amount is secreted into the medium from which it can be purified following established procedures. The cell-associated insoluble protein can be solubilized in 6 M urea after breaking up the cells by sonication. Renaturation is achieved by dilution or dialysis. Subsequent phosphocellulose chromatography yields a homogeneous protein preparation which is shown by a variety of biochemical and biophysical analyses to be indistinguishable from conventionally prepared material. The high yield (> 10 mg/500-ml culture) and the ease of preparation (2 to 3 days) make this an attractive alternative to previously described procedures. | lld:pubmed |
| pubmed-article:8167472 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8167472 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8167472 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8167472 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8167472 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8167472 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8167472 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:8167472 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8167472 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8167472 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8167472 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8167472 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:8167472 | pubmed:issn | 1046-5928 | lld:pubmed |
| pubmed-article:8167472 | pubmed:author | pubmed-author:PingoudAA | lld:pubmed |
| pubmed-article:8167472 | pubmed:author | pubmed-author:WendtFF | lld:pubmed |
| pubmed-article:8167472 | pubmed:author | pubmed-author:UrbankeCC | lld:pubmed |
| pubmed-article:8167472 | pubmed:author | pubmed-author:TholeHH | lld:pubmed |
| pubmed-article:8167472 | pubmed:author | pubmed-author:RüterTT | lld:pubmed |
| pubmed-article:8167472 | pubmed:author | pubmed-author:FriedhoffPP | lld:pubmed |
| pubmed-article:8167472 | pubmed:author | pubmed-author:GimadutdinowO... | lld:pubmed |
| pubmed-article:8167472 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8167472 | pubmed:volume | 5 | lld:pubmed |
| pubmed-article:8167472 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8167472 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8167472 | pubmed:pagination | 37-43 | lld:pubmed |
| pubmed-article:8167472 | pubmed:dateRevised | 2000-12-18 | lld:pubmed |
| pubmed-article:8167472 | pubmed:meshHeading | pubmed-meshheading:8167472-... | lld:pubmed |
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| pubmed-article:8167472 | pubmed:meshHeading | pubmed-meshheading:8167472-... | lld:pubmed |
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| pubmed-article:8167472 | pubmed:meshHeading | pubmed-meshheading:8167472-... | lld:pubmed |
| pubmed-article:8167472 | pubmed:meshHeading | pubmed-meshheading:8167472-... | lld:pubmed |
| pubmed-article:8167472 | pubmed:meshHeading | pubmed-meshheading:8167472-... | lld:pubmed |
| pubmed-article:8167472 | pubmed:meshHeading | pubmed-meshheading:8167472-... | lld:pubmed |
| pubmed-article:8167472 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:8167472 | pubmed:articleTitle | A procedure for renaturation and purification of the extracellular Serratia marcescens nuclease from genetically engineered Escherichia coli. | lld:pubmed |
| pubmed-article:8167472 | pubmed:affiliation | Institut für Biochemie, Fachbereich Biologie, Justus-Liebig-Universität, Giessen, Germany. | lld:pubmed |
| pubmed-article:8167472 | pubmed:publicationType | Journal Article | lld:pubmed |
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