| pubmed-article:8151292 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8151292 | lifeskim:mentions | umls-concept:C0206679 | lld:lifeskim |
| pubmed-article:8151292 | lifeskim:mentions | umls-concept:C0521009 | lld:lifeskim |
| pubmed-article:8151292 | lifeskim:mentions | umls-concept:C0080194 | lld:lifeskim |
| pubmed-article:8151292 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:8151292 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:8151292 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:8151292 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:8151292 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:8151292 | pubmed:dateCreated | 1994-5-9 | lld:pubmed |
| pubmed-article:8151292 | pubmed:abstractText | The DNA sequence of herpes simplex virus type 1 (HSV-1) strain 17+ in the region coding for the polypeptide ICP34.5 predicts a protein of 248 amino acids with a proposed M(r) of 26,158. The entire RL1 open reading frame was cloned into the expression vector pET8c to enable over-expression of ICP34.5 in Escherichia coli. The expressed protein was partially purified and used as an immunogen to produce a polyclonal antiserum in rabbits. Construction of an ICP34.5 null mutant (1771), demonstrated that the predicted open reading frame for ICP34.5 in strain 17+ is correct and confirmed that HSV-1 strain 17+ ICP34.5 specifically determines neurovirulence. The specificity of the anti-serum directed against the E. coli-expressed ICP34.5 was defined by Western blotting of wild-type and RL1-negative infected cell extracts. | lld:pubmed |
| pubmed-article:8151292 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8151292 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8151292 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8151292 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8151292 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8151292 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8151292 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8151292 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8151292 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:8151292 | pubmed:issn | 0022-1317 | lld:pubmed |
| pubmed-article:8151292 | pubmed:author | pubmed-author:BrownS MSM | lld:pubmed |
| pubmed-article:8151292 | pubmed:author | pubmed-author:MacLeanA RAR | lld:pubmed |
| pubmed-article:8151292 | pubmed:author | pubmed-author:HopeR GRG | lld:pubmed |
| pubmed-article:8151292 | pubmed:author | pubmed-author:McKieE AEA | lld:pubmed |
| pubmed-article:8151292 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8151292 | pubmed:volume | 75 ( Pt 4) | lld:pubmed |
| pubmed-article:8151292 | pubmed:geneSymbol | RL1 | lld:pubmed |
| pubmed-article:8151292 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8151292 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8151292 | pubmed:pagination | 733-41 | lld:pubmed |
| pubmed-article:8151292 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:8151292 | pubmed:meshHeading | pubmed-meshheading:8151292-... | lld:pubmed |
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| pubmed-article:8151292 | pubmed:meshHeading | pubmed-meshheading:8151292-... | lld:pubmed |
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| pubmed-article:8151292 | pubmed:meshHeading | pubmed-meshheading:8151292-... | lld:pubmed |
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| pubmed-article:8151292 | pubmed:meshHeading | pubmed-meshheading:8151292-... | lld:pubmed |
| pubmed-article:8151292 | pubmed:meshHeading | pubmed-meshheading:8151292-... | lld:pubmed |
| pubmed-article:8151292 | pubmed:meshHeading | pubmed-meshheading:8151292-... | lld:pubmed |
| pubmed-article:8151292 | pubmed:meshHeading | pubmed-meshheading:8151292-... | lld:pubmed |
| pubmed-article:8151292 | pubmed:meshHeading | pubmed-meshheading:8151292-... | lld:pubmed |
| pubmed-article:8151292 | pubmed:meshHeading | pubmed-meshheading:8151292-... | lld:pubmed |
| pubmed-article:8151292 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:8151292 | pubmed:articleTitle | Characterization of the herpes simplex virus type 1 strain 17+ neurovirulence gene RL1 and its expression in a bacterial system. | lld:pubmed |
| pubmed-article:8151292 | pubmed:affiliation | MRC Virology Unit, Institute of Virology, Glasgow, U.K. | lld:pubmed |
| pubmed-article:8151292 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8151292 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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