| pubmed-article:8134382 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8134382 | lifeskim:mentions | umls-concept:C0443286 | lld:lifeskim |
| pubmed-article:8134382 | lifeskim:mentions | umls-concept:C0036849 | lld:lifeskim |
| pubmed-article:8134382 | lifeskim:mentions | umls-concept:C1709694 | lld:lifeskim |
| pubmed-article:8134382 | lifeskim:mentions | umls-concept:C1708929 | lld:lifeskim |
| pubmed-article:8134382 | lifeskim:mentions | umls-concept:C1561491 | lld:lifeskim |
| pubmed-article:8134382 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:8134382 | pubmed:dateCreated | 1994-4-21 | lld:pubmed |
| pubmed-article:8134382 | pubmed:abstractText | Automated instruments for DNA sequencing greatly simplify data collection in the Sanger sequencing procedure. By contrast, the so-called front-end problems of preparing sequencing templates, performing sequencing reactions, and loading these on the instruments remain major obstacles to extensive sequencing projects. We describe here the use of a manifold support to prepare and perform sequencing reactions on large sets of templates in parallel, as well as to load the reaction products on a sequencing instrument. In this manner, all reaction steps are performed without pipetting the samples. The strategy is applied to sequencing PCR-amplified clones of the human mitochondrial D-loop and for detection of heterozygous positions in the human major histocompatibility complex class II gene HLA-DQB, amplified from genomic DNA samples. This technique will promote sequencing in a clinical context and could form the basis of more efficient genomic sequencing strategies. | lld:pubmed |
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| pubmed-article:8134382 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8134382 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8134382 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:8134382 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8134382 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:8134382 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:8134382 | pubmed:author | pubmed-author:StewartJJ | lld:pubmed |
| pubmed-article:8134382 | pubmed:author | pubmed-author:LandegrenUU | lld:pubmed |
| pubmed-article:8134382 | pubmed:author | pubmed-author:Lagerström-Fe... | lld:pubmed |
| pubmed-article:8134382 | pubmed:author | pubmed-author:LagerkvistAA | lld:pubmed |
| pubmed-article:8134382 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8134382 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:8134382 | pubmed:volume | 91 | lld:pubmed |
| pubmed-article:8134382 | pubmed:geneSymbol | HLA-DQB | lld:pubmed |
| pubmed-article:8134382 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8134382 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8134382 | pubmed:pagination | 2245-9 | lld:pubmed |
| pubmed-article:8134382 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:8134382 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:8134382 | pubmed:articleTitle | Manifold sequencing: efficient processing of large sets of sequencing reactions. | lld:pubmed |
| pubmed-article:8134382 | pubmed:affiliation | Beijer Laboratory, Department of Medical Genetics, Uppsala Biomedical Center, Sweden. | lld:pubmed |
| pubmed-article:8134382 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8134382 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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