| pubmed-article:8051693 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8051693 | lifeskim:mentions | umls-concept:C0007452 | lld:lifeskim |
| pubmed-article:8051693 | lifeskim:mentions | umls-concept:C0376604 | lld:lifeskim |
| pubmed-article:8051693 | lifeskim:mentions | umls-concept:C0086376 | lld:lifeskim |
| pubmed-article:8051693 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:8051693 | lifeskim:mentions | umls-concept:C0086597 | lld:lifeskim |
| pubmed-article:8051693 | lifeskim:mentions | umls-concept:C0234112 | lld:lifeskim |
| pubmed-article:8051693 | lifeskim:mentions | umls-concept:C0439799 | lld:lifeskim |
| pubmed-article:8051693 | lifeskim:mentions | umls-concept:C0521116 | lld:lifeskim |
| pubmed-article:8051693 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:8051693 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:8051693 | pubmed:dateCreated | 1994-9-8 | lld:pubmed |
| pubmed-article:8051693 | pubmed:abstractText | The effects of G-protein activation were investigated on tonic, large depolarization-induced Ca2+ channel facilitation in cultured bovine adrenal chromaffin cells. Under whole-cell voltage clamp, activation of G proteins by intracellular dialysis with 200 microM GTP-gamma S did not significantly affect prepulse facilitation or whole-cell Ba2+ current (IBa) density. In contrast, inactivation of G proteins by intracellular GDP-beta S or pertussis toxin (PTX) pretreatment completely abolished or markedly attenuated facilitation of IBa, respectively. GDP-beta S dialysis resulted in nearly a threefold increase in peak IBa density, whereas PTX pretreatment resulted in a 50% increase. Our results indicate that under control recording conditions (200 microM intracellular GTP), G proteins are tonically activated and suppress high-voltage-activated (HVA) Ca2+ channels in a voltage-dependent and voltage-independent manner. Local superfusion of chromaffin cells with normal bath solution produced a rapid and reversible increase (approximately 50%) in IBa amplitudes that also abolished prepulse facilitation. Together, these results demonstrate that tonic facilitation of HVA Ca2+ channels in bovine chromaffin cells involves the voltage-dependent relief of a G-protein-mediated suppression, imposed by chromaffin cell secretory products that feedback and activate G-protein-coupled autoreceptors. | lld:pubmed |
| pubmed-article:8051693 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8051693 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8051693 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8051693 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8051693 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:8051693 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8051693 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:8051693 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8051693 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8051693 | pubmed:month | May | lld:pubmed |
| pubmed-article:8051693 | pubmed:issn | 0022-2631 | lld:pubmed |
| pubmed-article:8051693 | pubmed:author | pubmed-author:PunR YRY | lld:pubmed |
| pubmed-article:8051693 | pubmed:author | pubmed-author:DoupnikC ACA | lld:pubmed |
| pubmed-article:8051693 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8051693 | pubmed:volume | 140 | lld:pubmed |
| pubmed-article:8051693 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8051693 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8051693 | pubmed:pagination | 47-56 | lld:pubmed |
| pubmed-article:8051693 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:8051693 | pubmed:meshHeading | pubmed-meshheading:8051693-... | lld:pubmed |
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| pubmed-article:8051693 | pubmed:meshHeading | pubmed-meshheading:8051693-... | lld:pubmed |
| pubmed-article:8051693 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:8051693 | pubmed:articleTitle | G-protein activation mediates prepulse facilitation of Ca2+ channel currents in bovine chromaffin cells. | lld:pubmed |
| pubmed-article:8051693 | pubmed:affiliation | Department of Physiology and Biophysics, University of Cincinnati College of Medicine, Ohio 45267-0576. | lld:pubmed |
| pubmed-article:8051693 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8051693 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8051693 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:8051693 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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