| pubmed-article:8051391 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8051391 | lifeskim:mentions | umls-concept:C0019169 | lld:lifeskim |
| pubmed-article:8051391 | lifeskim:mentions | umls-concept:C0019163 | lld:lifeskim |
| pubmed-article:8051391 | lifeskim:mentions | umls-concept:C0037140 | lld:lifeskim |
| pubmed-article:8051391 | lifeskim:mentions | umls-concept:C0030705 | lld:lifeskim |
| pubmed-article:8051391 | lifeskim:mentions | umls-concept:C0039195 | lld:lifeskim |
| pubmed-article:8051391 | lifeskim:mentions | umls-concept:C0524909 | lld:lifeskim |
| pubmed-article:8051391 | lifeskim:mentions | umls-concept:C1704632 | lld:lifeskim |
| pubmed-article:8051391 | lifeskim:mentions | umls-concept:C0871261 | lld:lifeskim |
| pubmed-article:8051391 | lifeskim:mentions | umls-concept:C0205178 | lld:lifeskim |
| pubmed-article:8051391 | lifeskim:mentions | umls-concept:C2911692 | lld:lifeskim |
| pubmed-article:8051391 | lifeskim:mentions | umls-concept:C1706817 | lld:lifeskim |
| pubmed-article:8051391 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:8051391 | pubmed:dateCreated | 1994-9-6 | lld:pubmed |
| pubmed-article:8051391 | pubmed:abstractText | An in vitro model was developed that allowed the analysis of hepatitis B virus-specific cytotoxic T lymphocyte responses in patients suffering from acute and chronic hepatitis B virus infections. Since virus-specific cytotoxic T lymphocytes recognize endogenously synthesized and processed antigen only when it is presented in the context of autologous HLA class I molecules and since hepatitis B virus does not infect human cells in vitro, a panel of recombinant vaccinia viruses was constructed to induce the expression of hepatitis B virus envelope and nucleocapsid proteins in cultured primary cells or cell lines derived from the patients to be studied. In order for a cytotoxic T lymphocyte response to be detectable with the currently available techniques, a sufficient number of activated cytotoxic T lymphocytes is required. To meet this requirement, lymphocytes freshly isolated from venous blood were stimulated in vitro with recombinant vaccinia-infected and formaldehyde-fixed autologous T lymphoblasts. The presence of hepatitis B virus-specific cytotoxic T lymphocytes, amplified and activated during this induction culture, was demonstrated in a microcytotoxicity assay using 51Cr-labeled, recombinant vaccinia-infected Epstein-Barr virus-immortalized, autologous B lymphocytes as target cells. Using this in vitro model, we were able to demonstrate the presence of hepatitis B virus envelope- and nucleocapsid-specific cytotoxic T lymphocytes in venous blood from one subject who had recently recovered from an acute hepatitis B virus infection and in three patients suffering from chronic hepatitis B virus infections. No hepatitis B virus-specific cytotoxic T lymphocytes activity was discernible in the venous blood from two vaccine recipients.(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
| pubmed-article:8051391 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8051391 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8051391 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8051391 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8051391 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:8051391 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8051391 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8051391 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:8051391 | pubmed:issn | 0168-8278 | lld:pubmed |
| pubmed-article:8051391 | pubmed:author | pubmed-author:PallGG | lld:pubmed |
| pubmed-article:8051391 | pubmed:author | pubmed-author:Van HeckeEE | lld:pubmed |
| pubmed-article:8051391 | pubmed:author | pubmed-author:BastinCC | lld:pubmed |
| pubmed-article:8051391 | pubmed:author | pubmed-author:Leroux-RoelsG... | lld:pubmed |
| pubmed-article:8051391 | pubmed:author | pubmed-author:SlaouiMM | lld:pubmed |
| pubmed-article:8051391 | pubmed:author | pubmed-author:MolitorCC | lld:pubmed |
| pubmed-article:8051391 | pubmed:author | pubmed-author:ParadijsJJ | lld:pubmed |
| pubmed-article:8051391 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8051391 | pubmed:volume | 20 | lld:pubmed |
| pubmed-article:8051391 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8051391 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8051391 | pubmed:pagination | 514-23 | lld:pubmed |
| pubmed-article:8051391 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:8051391 | pubmed:meshHeading | pubmed-meshheading:8051391-... | lld:pubmed |
| pubmed-article:8051391 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:8051391 | pubmed:articleTitle | Hepatitis B virus-specific cytotoxic T lymphocyte responses in patients with acute and chronic hepatitis B virus infection. | lld:pubmed |
| pubmed-article:8051391 | pubmed:affiliation | Department of Clinical Chemistry, University of Gent, Belgium. | lld:pubmed |
| pubmed-article:8051391 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8051391 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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