| pubmed-article:8031142 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8031142 | lifeskim:mentions | umls-concept:C0007589 | lld:lifeskim |
| pubmed-article:8031142 | lifeskim:mentions | umls-concept:C0949771 | lld:lifeskim |
| pubmed-article:8031142 | lifeskim:mentions | umls-concept:C0456387 | lld:lifeskim |
| pubmed-article:8031142 | lifeskim:mentions | umls-concept:C1511938 | lld:lifeskim |
| pubmed-article:8031142 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:8031142 | pubmed:dateCreated | 1994-8-11 | lld:pubmed |
| pubmed-article:8031142 | pubmed:abstractText | Injection of mRNA from GF-14 cells (a mouse lymphocyte cell line) into Xenopus oocytes led to the expression of a transport activity characteristic of amino acid transport system A, i.e., Na+ dependent and recognizing aminoisobutyric acid and its methyl derivative (AIB and meAIB). Sucrose density gradient fractionation of GF-14 mRNA showed that the maximum level of expression of activity was associated with a 2.2-kb RNA fraction. Pretreatment of GF-14 cells with insulin caused a twofold increase in system A transport activity in the cells themselves and the mRNA from the insulin-treated cells induced a comparable increase in A system transport over control mRNA when expressed in oocytes. mRNA from cells treated with insulin in presence of actinomycin D did not show a response to insulin, suggesting that GF-14 cells synthesized new transporter in response to insulin. Similar experiments with mRNA from the Ehrlich ascites cells, showed that expression of system A type transport was associated chiefly with a 4.2-kb mRNA fraction. Although insulin treatment of Ehrlich cells also caused an increase in A system transport activity in the cells themselves (nearly twofold), the response to insulin was not blocked by actinomycin D or cycloheximide. Moreover, mRNA from insulin-treated and untreated Ehrlich cells gave the same level of expression in oocytes of A type amino acid transport. In contrast to GF-14 cells, Western blots of plasma membranes showed that insulin treatment of Ehrlich cells increased the amount therein of a 120- to 130-kDa peptide, previously associated with amino acid transport (Proc. Natl. Acad. Sci. USA 85, 7877, 1988), suggesting that in the Ehrlich cells, but not in GF-14, cells this polypeptide was translocated from intracellular sites in response to insulin. The data associate two distinctly different responses to insulin with A-type amino acid transporters and are consistent with the existence of more than a single A type amino acid transporter in mouse cells. | lld:pubmed |
| pubmed-article:8031142 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8031142 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8031142 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:8031142 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:8031142 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8031142 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8031142 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:8031142 | pubmed:issn | 0003-9861 | lld:pubmed |
| pubmed-article:8031142 | pubmed:author | pubmed-author:JohnstoneR... | lld:pubmed |
| pubmed-article:8031142 | pubmed:author | pubmed-author:LiuNN | lld:pubmed |
| pubmed-article:8031142 | pubmed:author | pubmed-author:McCormickJ... | lld:pubmed |
| pubmed-article:8031142 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8031142 | pubmed:volume | 312 | lld:pubmed |
| pubmed-article:8031142 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8031142 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8031142 | pubmed:pagination | 308-15 | lld:pubmed |
| pubmed-article:8031142 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:8031142 | pubmed:meshHeading | pubmed-meshheading:8031142-... | lld:pubmed |
| pubmed-article:8031142 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:8031142 | pubmed:articleTitle | Differentiation of two classes of "A" system amino acid transporters. | lld:pubmed |
| pubmed-article:8031142 | pubmed:affiliation | Department of Biochemistry, McGill University, Montreal, Quebec, Canada. | lld:pubmed |
| pubmed-article:8031142 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8031142 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:8031142 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8031142 | lld:pubmed |
