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pubmed-article:7981909pubmed:abstractTextCircumstantial evidence from electron microscopic and immunological studies support the view that Paget's disease of bone represents a slow virus infection. However, there is only limited information available regarding its electron microscopic, enzyme and immunocytochemical characteristics. Two cases were studied using electron microscopy with particular emphasis on the inclusions in osteoclasts. Detailed ultrastructural and cytochemical studies including immuno-electron microscopy were performed. Some osteoclasts demonstrated specific virus-like structures composed of aggregations of microtubules in the nucleus and cytoplasm. The structures were easily digested by trypsin or protease, and were sensitive to RNase, which provided substantial evidence of a proteinaceous nature and inclusion of ribonucleic acid. Immunocytochemical examination identified binding of anti-respiratory syncytial virus and anti-measles virus antibodies in the tissue obtained from one of the two cases examined. The presence of viral antigens in structures in the cytoplasm of Pagetic osteoclasts supports the theory of paramyxovirus involvement in this disease.lld:pubmed
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pubmed-article:7981909pubmed:dateRevised2004-11-17lld:pubmed
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pubmed-article:7981909pubmed:articleTitleElectron microscopic evidence of a viral nature for osteoclast inclusions in Paget's disease of bone.lld:pubmed
pubmed-article:7981909pubmed:affiliationDepartment of Orthopaedic Surgery, Nara Medical University, Japan.lld:pubmed
pubmed-article:7981909pubmed:publicationTypeJournal Articlelld:pubmed
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