| pubmed-article:7968577 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7968577 | lifeskim:mentions | umls-concept:C0086045 | lld:lifeskim |
| pubmed-article:7968577 | lifeskim:mentions | umls-concept:C0026004 | lld:lifeskim |
| pubmed-article:7968577 | lifeskim:mentions | umls-concept:C1274040 | lld:lifeskim |
| pubmed-article:7968577 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:7968577 | lifeskim:mentions | umls-concept:C0026879 | lld:lifeskim |
| pubmed-article:7968577 | lifeskim:mentions | umls-concept:C0022877 | lld:lifeskim |
| pubmed-article:7968577 | lifeskim:mentions | umls-concept:C1442989 | lld:lifeskim |
| pubmed-article:7968577 | lifeskim:mentions | umls-concept:C0376249 | lld:lifeskim |
| pubmed-article:7968577 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:7968577 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:7968577 | pubmed:dateCreated | 1994-12-29 | lld:pubmed |
| pubmed-article:7968577 | pubmed:abstractText | In a previous collaborative work, we have recently shown that the cytochalasin-B (Cyt-B) concentration used in the human lymphocytes cytokinesis-block micronucleus (CBMN) assay is an important variable in the baseline micronuclei (MN) frequency as well as in the percentage of binucleated cells obtained. Now we have investigated how Cyt-B concentration modulates the MN frequency induced in whole blood human lymphocyte cultures by two clastogens (ethyl methanesulphonate and mitomycin-C) and two aneugens (colchicine and vincristine sulphate). The experimental design includes six donors, two concentrations of Cyt-B (3 and 6 micrograms/ml), two concentrations of the four chemicals tested and the exchange of slides between laboratories. The statistical analysis of the results shows: (i) non-significant differences in the MN frequencies and in the toxicity results between scorers from each laboratory, except for 0.06 microM colchicine at 3 micrograms/ml Cyt-B; (ii) an induction of MN by all genotoxic agents tested, the frequencies being lower with 6 than with 3 micrograms/ml Cyt-B, in control and aneugen-treated cultures; and (iii) significant differences between Cyt-B concentrations in several treatments, obtaining lower MN frequencies and higher values for nuclear division index and % binucleated cells when 6 micrograms/ml Cyt-B was used. Bearing in mind these results as well as the toxicity data showing that 6 micrograms/ml Cyt-B is much more effective in blocking cytokinesis, we can conclude that the use of 3 micrograms/ml Cyt-B may overestimate the induced frequency of MN. | lld:pubmed |
| pubmed-article:7968577 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7968577 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7968577 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7968577 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7968577 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7968577 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7968577 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7968577 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7968577 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7968577 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7968577 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:7968577 | pubmed:issn | 0267-8357 | lld:pubmed |
| pubmed-article:7968577 | pubmed:author | pubmed-author:TanzarellaCC | lld:pubmed |
| pubmed-article:7968577 | pubmed:author | pubmed-author:MarcosRR | lld:pubmed |
| pubmed-article:7968577 | pubmed:author | pubmed-author:AntocciaAA | lld:pubmed |
| pubmed-article:7968577 | pubmed:author | pubmed-author:CreusAA | lld:pubmed |
| pubmed-article:7968577 | pubmed:author | pubmed-author:XamenaNN | lld:pubmed |
| pubmed-article:7968577 | pubmed:author | pubmed-author:DegrassiFF | lld:pubmed |
| pubmed-article:7968577 | pubmed:author | pubmed-author:SurrallésJJ | lld:pubmed |
| pubmed-article:7968577 | pubmed:author | pubmed-author:PerinCC | lld:pubmed |
| pubmed-article:7968577 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7968577 | pubmed:volume | 9 | lld:pubmed |
| pubmed-article:7968577 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7968577 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7968577 | pubmed:pagination | 347-53 | lld:pubmed |
| pubmed-article:7968577 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:7968577 | pubmed:meshHeading | pubmed-meshheading:7968577-... | lld:pubmed |
| pubmed-article:7968577 | pubmed:meshHeading | pubmed-meshheading:7968577-... | lld:pubmed |
| pubmed-article:7968577 | pubmed:meshHeading | pubmed-meshheading:7968577-... | lld:pubmed |
| pubmed-article:7968577 | pubmed:meshHeading | pubmed-meshheading:7968577-... | lld:pubmed |
| pubmed-article:7968577 | pubmed:meshHeading | pubmed-meshheading:7968577-... | lld:pubmed |
| pubmed-article:7968577 | pubmed:meshHeading | pubmed-meshheading:7968577-... | lld:pubmed |
| pubmed-article:7968577 | pubmed:meshHeading | pubmed-meshheading:7968577-... | lld:pubmed |
| pubmed-article:7968577 | pubmed:meshHeading | pubmed-meshheading:7968577-... | lld:pubmed |
| pubmed-article:7968577 | pubmed:meshHeading | pubmed-meshheading:7968577-... | lld:pubmed |
| pubmed-article:7968577 | pubmed:meshHeading | pubmed-meshheading:7968577-... | lld:pubmed |
| pubmed-article:7968577 | pubmed:meshHeading | pubmed-meshheading:7968577-... | lld:pubmed |
| pubmed-article:7968577 | pubmed:meshHeading | pubmed-meshheading:7968577-... | lld:pubmed |
| pubmed-article:7968577 | pubmed:meshHeading | pubmed-meshheading:7968577-... | lld:pubmed |
| pubmed-article:7968577 | pubmed:meshHeading | pubmed-meshheading:7968577-... | lld:pubmed |
| pubmed-article:7968577 | pubmed:meshHeading | pubmed-meshheading:7968577-... | lld:pubmed |
| pubmed-article:7968577 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7968577 | pubmed:articleTitle | The effect of cytochalasin-B concentration on the frequency of micronuclei induced by four standard mutagens. Results from two laboratories. | lld:pubmed |
| pubmed-article:7968577 | pubmed:affiliation | Departament de Genètica i de Microbiologia, Edifici C, Universitat Autonoma de Barcelona, Bellaterra (Cerdanyola del Vallès), Spain. | lld:pubmed |
| pubmed-article:7968577 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7968577 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:7968577 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:7968577 | pubmed:publicationType | Multicenter Study | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7968577 | lld:pubmed |
