| pubmed-article:7962126 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7962126 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:7962126 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:7962126 | lifeskim:mentions | umls-concept:C0007603 | lld:lifeskim |
| pubmed-article:7962126 | lifeskim:mentions | umls-concept:C0178719 | lld:lifeskim |
| pubmed-article:7962126 | lifeskim:mentions | umls-concept:C0243144 | lld:lifeskim |
| pubmed-article:7962126 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:7962126 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:7962126 | lifeskim:mentions | umls-concept:C1698986 | lld:lifeskim |
| pubmed-article:7962126 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:7962126 | pubmed:dateCreated | 1994-12-27 | lld:pubmed |
| pubmed-article:7962126 | pubmed:abstractText | Experiments were undertaken to investigate the role of intracellular Ca2+ stores in the regulation of Ca2+ uptake in the cultured B-lymphocytic cell line CH12.LX.C4.5F5. Release of intracellular Ca2+ stores by addition of thapsigargin was accompanied by a biphasic increase in intracellular calcium concentration [Ca2+]i). The initial rise in [Ca2+]i was due to release of Ca2+ from intracellular stores as determined by its maintenance in the absence of extracellular Ca2+. The secondary phase was 1) dependent on the presence of extracellular Ca2+, 2) inhibited by 5 mM extracellular Ni2+, and 3) inhibited by high K+, consistent with electrogenic Ca2+ uptake from the extracellular medium. In order to more accurately investigate the electrogenic nature of this pathway we measured the membrane potential changes accompanying Ca2+ influx stimulated by release of Ca2+ from intracellular stores using bis(1,3-diethylthiobarbituric acid trimethine) oxonol in Bapta-loaded cells. Addition of 5 mM Ca2+ to cells pretreated with doses of thapsigargin or ionomycin shown to release intracellular Ca2+ stores induced a depolarization which was 1) dependent upon extracellular Ca2+, 2) abolished by 5 mM Ni2+, 3) independent of extracellular Na+, and 4) dependent upon Bapta loading. This depolarization was followed by a charybdotoxin-sensitive repolarization consistent with secondary activation of K+ channels. Changes in [Ca2+]i monitored under identical conditions were monitored fluorimetrically using indo-1 and were found to correlate with the changes in Em. On the basis of these data we conclude that an electrogenic Ca(2+)-permeable pathway exists in this B-lymphocytic cell line which is regulated by the degree of filling of an internal Ca(2+)-store. | lld:pubmed |
| pubmed-article:7962126 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7962126 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7962126 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7962126 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7962126 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7962126 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7962126 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7962126 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7962126 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7962126 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7962126 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7962126 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:7962126 | pubmed:issn | 0021-9541 | lld:pubmed |
| pubmed-article:7962126 | pubmed:author | pubmed-author:BostK LKL | lld:pubmed |
| pubmed-article:7962126 | pubmed:author | pubmed-author:MasonM JMJ | lld:pubmed |
| pubmed-article:7962126 | pubmed:author | pubmed-author:MarriottII | lld:pubmed |
| pubmed-article:7962126 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7962126 | pubmed:volume | 161 | lld:pubmed |
| pubmed-article:7962126 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7962126 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7962126 | pubmed:pagination | 441-8 | lld:pubmed |
| pubmed-article:7962126 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
| pubmed-article:7962126 | pubmed:meshHeading | pubmed-meshheading:7962126-... | lld:pubmed |
| pubmed-article:7962126 | pubmed:meshHeading | pubmed-meshheading:7962126-... | lld:pubmed |
| pubmed-article:7962126 | pubmed:meshHeading | pubmed-meshheading:7962126-... | lld:pubmed |
| pubmed-article:7962126 | pubmed:meshHeading | pubmed-meshheading:7962126-... | lld:pubmed |
| pubmed-article:7962126 | pubmed:meshHeading | pubmed-meshheading:7962126-... | lld:pubmed |
| pubmed-article:7962126 | pubmed:meshHeading | pubmed-meshheading:7962126-... | lld:pubmed |
| pubmed-article:7962126 | pubmed:meshHeading | pubmed-meshheading:7962126-... | lld:pubmed |
| pubmed-article:7962126 | pubmed:meshHeading | pubmed-meshheading:7962126-... | lld:pubmed |
| pubmed-article:7962126 | pubmed:meshHeading | pubmed-meshheading:7962126-... | lld:pubmed |
| pubmed-article:7962126 | pubmed:meshHeading | pubmed-meshheading:7962126-... | lld:pubmed |
| pubmed-article:7962126 | pubmed:meshHeading | pubmed-meshheading:7962126-... | lld:pubmed |
| pubmed-article:7962126 | pubmed:meshHeading | pubmed-meshheading:7962126-... | lld:pubmed |
| pubmed-article:7962126 | pubmed:meshHeading | pubmed-meshheading:7962126-... | lld:pubmed |
| pubmed-article:7962126 | pubmed:meshHeading | pubmed-meshheading:7962126-... | lld:pubmed |
| pubmed-article:7962126 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7962126 | pubmed:articleTitle | Role of intracellular Ca2+ stores in the regulation of electrogenic plasma membrane Ca2+ uptake in a B-lymphocytic cell line. | lld:pubmed |
| pubmed-article:7962126 | pubmed:affiliation | Department of Physiology, Tulane University School of Medicine, New Orleans, Louisiana 70112. | lld:pubmed |
| pubmed-article:7962126 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7962126 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:7962126 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
