| pubmed-article:7925656 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7925656 | lifeskim:mentions | umls-concept:C0330390 | lld:lifeskim |
| pubmed-article:7925656 | lifeskim:mentions | umls-concept:C0007589 | lld:lifeskim |
| pubmed-article:7925656 | lifeskim:mentions | umls-concept:C0206664 | lld:lifeskim |
| pubmed-article:7925656 | lifeskim:mentions | umls-concept:C1414685 | lld:lifeskim |
| pubmed-article:7925656 | lifeskim:mentions | umls-concept:C0205421 | lld:lifeskim |
| pubmed-article:7925656 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:7925656 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:7925656 | pubmed:dateCreated | 1994-11-4 | lld:pubmed |
| pubmed-article:7925656 | pubmed:abstractText | We have investigated the retinoic acid-mediated activation of the transcriptional regulator HNF-3 beta during differentiation of mouse F9 embryonal carcinoma cells. Using gel shifts, HNF-3 beta DNA binding activity was clearly detected in differentiated cells, while F9 stem cells were devoid of this activity. We also demonstrated that HNF-3 beta mRNA is specific for differentiated cells. Addition of retinoic acid to F9 stem cells results in delayed activation of HNF-3 beta mRNA which can be detected 1-2 days after the initiation of differentiation. HNF-3 beta mRNA concentrations are maximal at approximately 4 days postdifferentiation and stay at elevated levels for at least 4 additional days. Nuclear run-on experiments clearly show that HNF-3 beta is activated at the level of transcriptional initiation, suggesting that the increases of beta-specific DNA binding activity and mRNA concentration are merely a reflection of this activation mechanism. F9 cells can give rise to three distinct differentiated cell types, visceral endoderm, parietal endoderm, and primitive endoderm, and we have observed HNF-3 beta stimulation during the formation of all three tissues. HNF-3 beta stimulation upon visceral endoderm differentiation is accompanied by the activation of HNF-3 target genes such as transthyretin, suggesting that HNF-3 beta is involved in the developmental activation of this gene. In contrast, HNF-3 beta target genes in parietal and primitive endoderm have yet to be identified. However, the stimulation of HNF-3 beta during primitive endoderm formation, which is an extremely early event during murine embryogenesis, points toward a role for the factor in crucial determination processes that occur early during development. | lld:pubmed |
| pubmed-article:7925656 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7925656 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7925656 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7925656 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7925656 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7925656 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7925656 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7925656 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7925656 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7925656 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7925656 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7925656 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:7925656 | pubmed:issn | 0014-4827 | lld:pubmed |
| pubmed-article:7925656 | pubmed:author | pubmed-author:JacobAA | lld:pubmed |
| pubmed-article:7925656 | pubmed:author | pubmed-author:ReichelR RRR | lld:pubmed |
| pubmed-article:7925656 | pubmed:author | pubmed-author:BudhirajaSS | lld:pubmed |
| pubmed-article:7925656 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7925656 | pubmed:volume | 214 | lld:pubmed |
| pubmed-article:7925656 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7925656 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7925656 | pubmed:pagination | 634-41 | lld:pubmed |
| pubmed-article:7925656 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:7925656 | pubmed:meshHeading | pubmed-meshheading:7925656-... | lld:pubmed |
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| pubmed-article:7925656 | pubmed:meshHeading | pubmed-meshheading:7925656-... | lld:pubmed |
| pubmed-article:7925656 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7925656 | pubmed:articleTitle | Delayed activation of HNF-3 beta upon retinoic acid-induced teratocarcinoma cell differentiation. | lld:pubmed |
| pubmed-article:7925656 | pubmed:affiliation | Department of Pharmacology and Molecular Biology, Chicago Medical School, Illinois 60064. | lld:pubmed |
| pubmed-article:7925656 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7925656 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:15376 | entrezgene:pubmed | pubmed-article:7925656 | lld:entrezgene |
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