| pubmed-article:7907303 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7907303 | lifeskim:mentions | umls-concept:C0041348 | lld:lifeskim |
| pubmed-article:7907303 | lifeskim:mentions | umls-concept:C0949765 | lld:lifeskim |
| pubmed-article:7907303 | lifeskim:mentions | umls-concept:C1514562 | lld:lifeskim |
| pubmed-article:7907303 | lifeskim:mentions | umls-concept:C0600448 | lld:lifeskim |
| pubmed-article:7907303 | lifeskim:mentions | umls-concept:C0205214 | lld:lifeskim |
| pubmed-article:7907303 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:7907303 | pubmed:dateCreated | 1994-4-8 | lld:pubmed |
| pubmed-article:7907303 | pubmed:abstractText | In trypanosomes, the generation of monocistronic mRNAs from polycistronic precursors is achieved via RNA processing, namely trans-splicing of the spliced leader sequence at the 5' end and cleavage/polyadenylation at the 3' end of the mRNA coding region. Recent evidence raised the intriguing possibility that these two reactions are coupled. To begin a dissection of the signals required for mRNA 5'-end and 3'-end formation and to uncover potential interactions between trans-splicing and polyadenylation, we mutagenized the intergenic region between the beta- and alpha-tubulin genes of Trypanosoma brucei. Block substitutions identified the pyrimidine-rich sequences at the alpha-tubulin 3'-splice-acceptor site as a major determinant for accurate trans-splicing downstream and 3'-end formation upstream. In addition to the utilization of cryptic 3'-splice sites, obliteration of the polypyrimidine tracts led to aberrant poly(A)+ site choice, even in the presence of the wild-type poly(A)+ site and neighboring sequences. Taken together, these results indicate that the polypyrimidine-rich sequences act as a bifunctional element that affects RNA processing both upstream and downstream from itself. This is consistent with the possibility that the polypyrimidine tract is recognized by both the trans-splicing and polyadenylation machineries, either sequentially or simultaneously. | lld:pubmed |
| pubmed-article:7907303 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7907303 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7907303 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7907303 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:7907303 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7907303 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:7907303 | pubmed:issn | 0890-9369 | lld:pubmed |
| pubmed-article:7907303 | pubmed:author | pubmed-author:TschudiCC | lld:pubmed |
| pubmed-article:7907303 | pubmed:author | pubmed-author:UlluEE | lld:pubmed |
| pubmed-article:7907303 | pubmed:author | pubmed-author:MatthewsK RKR | lld:pubmed |
| pubmed-article:7907303 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7907303 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:7907303 | pubmed:volume | 8 | lld:pubmed |
| pubmed-article:7907303 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7907303 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7907303 | pubmed:pagination | 491-501 | lld:pubmed |
| pubmed-article:7907303 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:7907303 | pubmed:meshHeading | pubmed-meshheading:7907303-... | lld:pubmed |
| pubmed-article:7907303 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7907303 | pubmed:articleTitle | A common pyrimidine-rich motif governs trans-splicing and polyadenylation of tubulin polycistronic pre-mRNA in trypanosomes. | lld:pubmed |
| pubmed-article:7907303 | pubmed:affiliation | Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520-8022. | lld:pubmed |
| pubmed-article:7907303 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7907303 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7907303 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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