| pubmed-article:7889172 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7889172 | lifeskim:mentions | umls-concept:C0030705 | lld:lifeskim |
| pubmed-article:7889172 | lifeskim:mentions | umls-concept:C0034790 | lld:lifeskim |
| pubmed-article:7889172 | lifeskim:mentions | umls-concept:C0339143 | lld:lifeskim |
| pubmed-article:7889172 | lifeskim:mentions | umls-concept:C0598666 | lld:lifeskim |
| pubmed-article:7889172 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:7889172 | lifeskim:mentions | umls-concept:C0457083 | lld:lifeskim |
| pubmed-article:7889172 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:7889172 | pubmed:dateCreated | 1995-4-20 | lld:pubmed |
| pubmed-article:7889172 | pubmed:abstractText | To date, it has remained unclear whether orbit-infiltrating T cells in patients with Graves' ophthalmopathy (GO) represent a primary immune response in which a limited number of T cell clones driving the disease are activated against specific antigens, or whether they participate in a non-specific inflammatory process. To characterize these T cells at the molecular level, we examined the T cell antigen receptor (TcR) V gene repertoire in situ in retroorbital tissue specimens obtained from patients with early and late stages of clinically severe GO and from patients with non-GO orbital conditions. Ribonucleic acid extracted from orbital tissue and peripheral blood lymphocytes (PBL) was reverse transcribed and amplified using the polymerase chain reaction and 22 V alpha and 24 V beta gene-specific oligonucleotide primers. The resulting TcR V alpha and V beta transcripts were verified by Southern hybridization analysis using TcR C region-specific, digoxigenin-labeled oligonucleotide probes. Compared with matched PBL, the retroorbital TcR V alpha and V beta gene repertoire expressed was heterogeneous, but revealed marked restriction of V gene usage in samples derived from retroorbital connective tissue and extraocular muscle of all eight patients with severe GO of short duration studied. In contrast, greater diversity of the TcR V beta gene repertoire and loss of TcR V alpha gene restriction was noted in four patients with late GO undergoing reconstructive eye muscle surgery. Unrestricted TcR V gene usage was demonstrated in orbital tissue and PBL samples obtained from control subjects. These results suggest that retroorbital TcR V gene usage is variable but markedly restricted during the earlier stages of GO. With increasing disease duration, greater diversity of the TcR V gene repertoire appears to develop, and oligoclonality of the T cell response may be lost. Selection of patients with early stages of GO will be important when further dissecting TcR usage and antigen specificity of orbit-infiltrating T lymphocytes in GO. | lld:pubmed |
| pubmed-article:7889172 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7889172 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7889172 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7889172 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7889172 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7889172 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7889172 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:7889172 | pubmed:issn | 0804-4643 | lld:pubmed |
| pubmed-article:7889172 | pubmed:author | pubmed-author:ScribaP CPC | lld:pubmed |
| pubmed-article:7889172 | pubmed:author | pubmed-author:ErnstGG | lld:pubmed |
| pubmed-article:7889172 | pubmed:author | pubmed-author:BallS LSL | lld:pubmed |
| pubmed-article:7889172 | pubmed:author | pubmed-author:HeufelderA... | lld:pubmed |
| pubmed-article:7889172 | pubmed:author | pubmed-author:HerterichSS | lld:pubmed |
| pubmed-article:7889172 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7889172 | pubmed:volume | 132 | lld:pubmed |
| pubmed-article:7889172 | pubmed:geneSymbol | V&bgr; | lld:pubmed |
| pubmed-article:7889172 | pubmed:geneSymbol | V&agr; | lld:pubmed |
| pubmed-article:7889172 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7889172 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7889172 | pubmed:pagination | 266-77 | lld:pubmed |
| pubmed-article:7889172 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:7889172 | pubmed:meshHeading | pubmed-meshheading:7889172-... | lld:pubmed |
| pubmed-article:7889172 | pubmed:meshHeading | pubmed-meshheading:7889172-... | lld:pubmed |
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| pubmed-article:7889172 | pubmed:meshHeading | pubmed-meshheading:7889172-... | lld:pubmed |
| pubmed-article:7889172 | pubmed:meshHeading | pubmed-meshheading:7889172-... | lld:pubmed |
| pubmed-article:7889172 | pubmed:meshHeading | pubmed-meshheading:7889172-... | lld:pubmed |
| pubmed-article:7889172 | pubmed:meshHeading | pubmed-meshheading:7889172-... | lld:pubmed |
| pubmed-article:7889172 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:7889172 | pubmed:articleTitle | Analysis of retroorbital T cell antigen receptor variable region gene usage in patients with Graves' ophthalmopathy. | lld:pubmed |
| pubmed-article:7889172 | pubmed:affiliation | Molecular Thyroid Research Unit, Klinikum Innenstadt, Ludwig-Maximilians-Universität, München, Germany. | lld:pubmed |
| pubmed-article:7889172 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7889172 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7889172 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7889172 | lld:pubmed |
