| pubmed-article:7854327 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7854327 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:7854327 | lifeskim:mentions | umls-concept:C1383501 | lld:lifeskim |
| pubmed-article:7854327 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:7854327 | lifeskim:mentions | umls-concept:C0680730 | lld:lifeskim |
| pubmed-article:7854327 | lifeskim:mentions | umls-concept:C1148554 | lld:lifeskim |
| pubmed-article:7854327 | lifeskim:mentions | umls-concept:C2346927 | lld:lifeskim |
| pubmed-article:7854327 | lifeskim:mentions | umls-concept:C0209892 | lld:lifeskim |
| pubmed-article:7854327 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:7854327 | pubmed:dateCreated | 1995-3-15 | lld:pubmed |
| pubmed-article:7854327 | pubmed:abstractText | The magnesium buffer coefficient (BMg) was calculated for BC3H-1 cells from the rise in cytosolic Mg2+ activity observed when magnesium was released from ATP after iodoacetate (IAA) and NaCN treatment. The basal cytosolic Mg2+ activity (0.54 +/- 0.1 mM) measured with mag-fura-2 doubled when 4.54 mM magnesium was liberated from ATP: BMg was 12.9 indicating that a 1 mM increase in Mg2+ activity requires an addition of about 13 mM magnesium. The accuracy of this value depends on these assumptions: (a) all of the magnesium released from ATP stayed in the cells; (b) the rise in Mg2+ was not secondary to pH-induced changes in BMg; (c) mag-fura-2 measured Mg2+ and not Ca2+; and (d) the accuracy of the mag-fura-2 calibration. Total magnesium did not change in response to IAA/CN treatment, thus the change in Mg2+ activity reflected a redistribution of cell magnesium. pH changes induced by NH4Cl pulse and removal had little effect on Mg2+ activity and the changes were slower than and opposite to pH-induced changes in Ca2+ activity measured by fura-2. Ca2+ responses were temporally uncoupled from Mg2+ responses when the cells were treated with IAA only and in no cases did Ca2+ levels rise above 1 microM, showing that the mag-fura-2 is responding to Mg2+. Additional studies demonstrated that approximately 90% of the mag-fura-2 signal was cytosolic in origin. The remaining non-diffusible mag-fura-2 either was bound to cytosolic membranes or sequestered in organelles with the fluorescence characteristics of the Mg2(+)-complexed form, even when cytosolic free Mg2+ activity was approximately 0.5 mM. This bound mag-fura-2 would appear to increase the Kd and thus clearly limits the accuracy of our estimmate for BMg. Despite this limitation, we demonstrate that Mg2+ is tightly regulated in face of large changes in extracellular Mg2+, and that interplay observed between pH, Ca2+ and Mg2+ activities strongly supports the hypothesis that these factors interact through a shared buffer capacity of the cell. | lld:pubmed |
| pubmed-article:7854327 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7854327 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7854327 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7854327 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:7854327 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7854327 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:7854327 | pubmed:issn | 0300-8177 | lld:pubmed |
| pubmed-article:7854327 | pubmed:author | pubmed-author:WalterAA | lld:pubmed |
| pubmed-article:7854327 | pubmed:author | pubmed-author:GrubbsR DRD | lld:pubmed |
| pubmed-article:7854327 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7854327 | pubmed:day | 13 | lld:pubmed |
| pubmed-article:7854327 | pubmed:volume | 136 | lld:pubmed |
| pubmed-article:7854327 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7854327 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7854327 | pubmed:pagination | 11-22 | lld:pubmed |
| pubmed-article:7854327 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:7854327 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7854327 | pubmed:articleTitle | Determination of cytosolic Mg2+ activity and buffering in BC3H-1 cells with mag-fura-2. | lld:pubmed |
| pubmed-article:7854327 | pubmed:affiliation | Department of Pharmacology and Toxicology, School of Medicine, Wright State University, Dayton, Ohio 45435. | lld:pubmed |
| pubmed-article:7854327 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7854327 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:7854327 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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