| pubmed-article:7785767 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7785767 | lifeskim:mentions | umls-concept:C0007303 | lld:lifeskim |
| pubmed-article:7785767 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:7785767 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:7785767 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:7785767 | pubmed:dateCreated | 1995-7-20 | lld:pubmed |
| pubmed-article:7785767 | pubmed:abstractText | UDP-D-Galactose:D-xylose galactosyltransferase (Galactosyltransferase-I) is an enzyme involved in the synthesis of the linkage region of chondroitin sulfate. Measurement of galactosyltransferase-I in mature articular cartilage has depended on milling such tissue in liquid nitrogen to break open the cells and enable interaction of exogenous substrates with galactosyltransferase-I. This process requires large amounts (approximately 1 g wet wt) of cartilage. This paper reports the development of an assay for galactosyltransferase-I in articular cartilage where 10-25 mg (wet wt) of the tissue was extracted with a buffer containing the detergent Thesit. This assay was more time efficient, required less tissue, and was linear for up to 6 h. It was effective on both mature bovine articular cartilage and embryonic chick epiphyseal cartilage, detecting 100 and 68% of total galactosyltransferase-I activity, respectively. The higher number of measurements available allowed kinetic studies to be conducted on bovine articular cartilage explant cultures under conditions of up- and down-regulation. Incubation with fetal calf serum resulted in an increase in galactosyltransferase-I activity. Galactosyltransferase-I activity, however, did not change markedly in cartilage cultured in the absence of fetal calf serum or in the presence of cycloheximide. A soluble preparation of galactosyltransferase-I, extracted from bovine articular cartilage using Thesit, eluted from a Superose-6 column, with a molecular mass of about 55 kDa. | lld:pubmed |
| pubmed-article:7785767 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7785767 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7785767 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7785767 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7785767 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7785767 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7785767 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7785767 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7785767 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7785767 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7785767 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7785767 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:7785767 | pubmed:issn | 0003-2697 | lld:pubmed |
| pubmed-article:7785767 | pubmed:author | pubmed-author:RobinsonH CHC | lld:pubmed |
| pubmed-article:7785767 | pubmed:author | pubmed-author:HandleyC JCJ | lld:pubmed |
| pubmed-article:7785767 | pubmed:author | pubmed-author:ParkerG JGJ | lld:pubmed |
| pubmed-article:7785767 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7785767 | pubmed:day | 20 | lld:pubmed |
| pubmed-article:7785767 | pubmed:volume | 226 | lld:pubmed |
| pubmed-article:7785767 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7785767 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7785767 | pubmed:pagination | 154-60 | lld:pubmed |
| pubmed-article:7785767 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:7785767 | pubmed:meshHeading | pubmed-meshheading:7785767-... | lld:pubmed |
| pubmed-article:7785767 | pubmed:meshHeading | pubmed-meshheading:7785767-... | lld:pubmed |
| pubmed-article:7785767 | pubmed:meshHeading | pubmed-meshheading:7785767-... | lld:pubmed |
| pubmed-article:7785767 | pubmed:meshHeading | pubmed-meshheading:7785767-... | lld:pubmed |
| pubmed-article:7785767 | pubmed:meshHeading | pubmed-meshheading:7785767-... | lld:pubmed |
| pubmed-article:7785767 | pubmed:meshHeading | pubmed-meshheading:7785767-... | lld:pubmed |
| pubmed-article:7785767 | pubmed:meshHeading | pubmed-meshheading:7785767-... | lld:pubmed |
| pubmed-article:7785767 | pubmed:meshHeading | pubmed-meshheading:7785767-... | lld:pubmed |
| pubmed-article:7785767 | pubmed:meshHeading | pubmed-meshheading:7785767-... | lld:pubmed |
| pubmed-article:7785767 | pubmed:meshHeading | pubmed-meshheading:7785767-... | lld:pubmed |
| pubmed-article:7785767 | pubmed:meshHeading | pubmed-meshheading:7785767-... | lld:pubmed |
| pubmed-article:7785767 | pubmed:meshHeading | pubmed-meshheading:7785767-... | lld:pubmed |
| pubmed-article:7785767 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:7785767 | pubmed:articleTitle | An assay for galactosyltransferase-I activity in articular cartilage. | lld:pubmed |
| pubmed-article:7785767 | pubmed:affiliation | Department of Biochemistry, Monash University, Clayton, Victoria, Australia. | lld:pubmed |
| pubmed-article:7785767 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7785767 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
