| pubmed-article:7745244 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7745244 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:7745244 | lifeskim:mentions | umls-concept:C0036849 | lld:lifeskim |
| pubmed-article:7745244 | lifeskim:mentions | umls-concept:C0599161 | lld:lifeskim |
| pubmed-article:7745244 | lifeskim:mentions | umls-concept:C1527177 | lld:lifeskim |
| pubmed-article:7745244 | lifeskim:mentions | umls-concept:C0441513 | lld:lifeskim |
| pubmed-article:7745244 | lifeskim:mentions | umls-concept:C0392762 | lld:lifeskim |
| pubmed-article:7745244 | lifeskim:mentions | umls-concept:C1524063 | lld:lifeskim |
| pubmed-article:7745244 | lifeskim:mentions | umls-concept:C0206415 | lld:lifeskim |
| pubmed-article:7745244 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:7745244 | pubmed:dateCreated | 1995-6-13 | lld:pubmed |
| pubmed-article:7745244 | pubmed:abstractText | We describe a simple and inexpensive method for the construction of multi-competitor molecules for use as internal standards in quantitative RT-PCR. The construction involves the linking and annealing of 20mer PCR primers with complementary 40mers using either a step-wise or bulk process. The entire construct is then ligated and amplified by PCR prior to cloning. Using this approach, we have constructed a gene containing priming sites for 18 different products of immunological interest, including murine cytokines and cell surface markers, as well as murine beta-actin and T. cruzi rRNA. The cost of production of the competitor is minimized by use of a high-throughput multi-oligonucleotide synthesizer for production of the individual components of the synthetic gene, and by use of the same oligonucleotides in gene construction and as primers for the RT-PCR reactions. This procedure can be applied to the production of other polycompetitor molecules as well as to the construction of other types of synthetic genes. | lld:pubmed |
| pubmed-article:7745244 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7745244 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7745244 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7745244 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7745244 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7745244 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7745244 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7745244 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7745244 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7745244 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:7745244 | pubmed:issn | 0022-1759 | lld:pubmed |
| pubmed-article:7745244 | pubmed:author | pubmed-author:McGrawR ARA | lld:pubmed |
| pubmed-article:7745244 | pubmed:author | pubmed-author:TarletonR LRL | lld:pubmed |
| pubmed-article:7745244 | pubmed:author | pubmed-author:HubbyBB | lld:pubmed |
| pubmed-article:7745244 | pubmed:author | pubmed-author:BenavidesG... | lld:pubmed |
| pubmed-article:7745244 | pubmed:author | pubmed-author:GrosseW MWM | lld:pubmed |
| pubmed-article:7745244 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7745244 | pubmed:day | 26 | lld:pubmed |
| pubmed-article:7745244 | pubmed:volume | 181 | lld:pubmed |
| pubmed-article:7745244 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7745244 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7745244 | pubmed:pagination | 145-56 | lld:pubmed |
| pubmed-article:7745244 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:7745244 | pubmed:meshHeading | pubmed-meshheading:7745244-... | lld:pubmed |
| pubmed-article:7745244 | pubmed:meshHeading | pubmed-meshheading:7745244-... | lld:pubmed |
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| pubmed-article:7745244 | pubmed:meshHeading | pubmed-meshheading:7745244-... | lld:pubmed |
| pubmed-article:7745244 | pubmed:meshHeading | pubmed-meshheading:7745244-... | lld:pubmed |
| pubmed-article:7745244 | pubmed:meshHeading | pubmed-meshheading:7745244-... | lld:pubmed |
| pubmed-article:7745244 | pubmed:meshHeading | pubmed-meshheading:7745244-... | lld:pubmed |
| pubmed-article:7745244 | pubmed:meshHeading | pubmed-meshheading:7745244-... | lld:pubmed |
| pubmed-article:7745244 | pubmed:meshHeading | pubmed-meshheading:7745244-... | lld:pubmed |
| pubmed-article:7745244 | pubmed:meshHeading | pubmed-meshheading:7745244-... | lld:pubmed |
| pubmed-article:7745244 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:7745244 | pubmed:articleTitle | Construction and use of a multi-competitor gene for quantitative RT-PCR using existing primer sets. | lld:pubmed |
| pubmed-article:7745244 | pubmed:affiliation | Department of Zoology, University of Georgia, Athens 30602, USA. | lld:pubmed |
| pubmed-article:7745244 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7745244 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7745244 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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