| pubmed-article:7733380 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7733380 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:7733380 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:7733380 | lifeskim:mentions | umls-concept:C0014124 | lld:lifeskim |
| pubmed-article:7733380 | lifeskim:mentions | umls-concept:C0005558 | lld:lifeskim |
| pubmed-article:7733380 | lifeskim:mentions | umls-concept:C0442016 | lld:lifeskim |
| pubmed-article:7733380 | lifeskim:mentions | umls-concept:C2339371 | lld:lifeskim |
| pubmed-article:7733380 | lifeskim:mentions | umls-concept:C0204727 | lld:lifeskim |
| pubmed-article:7733380 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:7733380 | lifeskim:mentions | umls-concept:C0205171 | lld:lifeskim |
| pubmed-article:7733380 | pubmed:issue | 4 Pt 2 | lld:pubmed |
| pubmed-article:7733380 | pubmed:dateCreated | 1995-6-1 | lld:pubmed |
| pubmed-article:7733380 | pubmed:abstractText | The study of adult human ventricular cells has been limited by tissue availability. In this study we describe techniques for the isolation of Ca(2+)-tolerant adult human ventricular cells from both transvenous endomyocardial and epicardial biopsies. Ca(2+)-tolerant cells were obtained from 80% of the biopsies processed. Although the yield of Ca(2+)-tolerant myocytes from either type of biopsy was low (1-5%), myocytes with normal resting potentials and action potentials can be obtained from single biopsy specimens, providing a source of normal human myocytes for electrophysiological study. Resting potentials (Vrest) were recorded in 41 isolated right ventricular endomyocardial cells at 37 degrees C. Sixteen cells were depolarized (Vrest = -26 +/- 13 mV), and 25 cells had normal resting potentials (Vrest = -84 +/- 6 mV). Action potentials were recorded in 16 cells. At a pacing cycle length of 1 s, 4 cells had prolonged action potential duration at 90% (APD90, 718 +/- 26 ms) and 10 cells had normal APD90 (381 +/- 94 ms) compared with those recorded from intact right ventricular septal trabeculae from explanted hearts. Voltage-clamp studies of isolated human ventricular myocytes obtained from these biopsies document the presence of currents previously reported from cells isolated from explanted hearts. | lld:pubmed |
| pubmed-article:7733380 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7733380 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7733380 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7733380 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7733380 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7733380 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7733380 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7733380 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7733380 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7733380 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:7733380 | pubmed:issn | 0002-9513 | lld:pubmed |
| pubmed-article:7733380 | pubmed:author | pubmed-author:BarryW HWH | lld:pubmed |
| pubmed-article:7733380 | pubmed:author | pubmed-author:PeetersG AGA | lld:pubmed |
| pubmed-article:7733380 | pubmed:author | pubmed-author:KarwandeS VSV | lld:pubmed |
| pubmed-article:7733380 | pubmed:author | pubmed-author:RenlundD GDG | lld:pubmed |
| pubmed-article:7733380 | pubmed:author | pubmed-author:SanguinettiM... | lld:pubmed |
| pubmed-article:7733380 | pubmed:author | pubmed-author:QiJ XJX | lld:pubmed |
| pubmed-article:7733380 | pubmed:author | pubmed-author:KonarzewskaHH | lld:pubmed |
| pubmed-article:7733380 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7733380 | pubmed:volume | 268 | lld:pubmed |
| pubmed-article:7733380 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7733380 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7733380 | pubmed:pagination | H1757-64 | lld:pubmed |
| pubmed-article:7733380 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:7733380 | pubmed:meshHeading | pubmed-meshheading:7733380-... | lld:pubmed |
| pubmed-article:7733380 | pubmed:meshHeading | pubmed-meshheading:7733380-... | lld:pubmed |
| pubmed-article:7733380 | pubmed:meshHeading | pubmed-meshheading:7733380-... | lld:pubmed |
| pubmed-article:7733380 | pubmed:meshHeading | pubmed-meshheading:7733380-... | lld:pubmed |
| pubmed-article:7733380 | pubmed:meshHeading | pubmed-meshheading:7733380-... | lld:pubmed |
| pubmed-article:7733380 | pubmed:meshHeading | pubmed-meshheading:7733380-... | lld:pubmed |
| pubmed-article:7733380 | pubmed:meshHeading | pubmed-meshheading:7733380-... | lld:pubmed |
| pubmed-article:7733380 | pubmed:meshHeading | pubmed-meshheading:7733380-... | lld:pubmed |
| pubmed-article:7733380 | pubmed:meshHeading | pubmed-meshheading:7733380-... | lld:pubmed |
| pubmed-article:7733380 | pubmed:meshHeading | pubmed-meshheading:7733380-... | lld:pubmed |
| pubmed-article:7733380 | pubmed:meshHeading | pubmed-meshheading:7733380-... | lld:pubmed |
| pubmed-article:7733380 | pubmed:meshHeading | pubmed-meshheading:7733380-... | lld:pubmed |
| pubmed-article:7733380 | pubmed:meshHeading | pubmed-meshheading:7733380-... | lld:pubmed |
| pubmed-article:7733380 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:7733380 | pubmed:articleTitle | Method for isolation of human ventricular myocytes from single endocardial and epicardial biopsies. | lld:pubmed |
| pubmed-article:7733380 | pubmed:affiliation | Division of Cardiology Surgery, University of Utah Medical Center, Salt Lake City, USA. | lld:pubmed |
| pubmed-article:7733380 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7733380 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7733380 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7733380 | lld:pubmed |
