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pubmed-article:7632820pubmed:abstractTextIntracellular free (ionized) magnesium concentration was measured in mononuclear cells isolated from healthy volunteers by use of dual wavelength fluorescence (indicator: mag-fura-2). We found a free Mg2+ concentration of 1.28 +/- 0.08 mmol/l in mononuclear cells isolated from heparinized blood. When we defibrinated blood samples prior to the isolation step we measured 0.78 +/- 0.05 mmol/l of free Mg2+ in these cells. We conclude that this difference is caused by the platelets present in the heparinized specimens.lld:pubmed
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pubmed-article:7632820pubmed:articleTitleDefibrination essential in the assay of ionized magnesium in mononuclear cells.lld:pubmed
pubmed-article:7632820pubmed:affiliationAfdeling Klinische Chemie van het Academisch Ziekenhuis Nijmegen, St. Radboud, Nijmegen, The Netherlands.lld:pubmed
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