| pubmed-article:762150 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:762150 | lifeskim:mentions | umls-concept:C0007452 | lld:lifeskim |
| pubmed-article:762150 | lifeskim:mentions | umls-concept:C0032008 | lld:lifeskim |
| pubmed-article:762150 | lifeskim:mentions | umls-concept:C0035696 | lld:lifeskim |
| pubmed-article:762150 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:762150 | lifeskim:mentions | umls-concept:C0071931 | lld:lifeskim |
| pubmed-article:762150 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:762150 | pubmed:dateCreated | 1979-4-28 | lld:pubmed |
| pubmed-article:762150 | pubmed:abstractText | Total cytoplasmic RNA, isolated from bovine anterior pituitary glands, directed the synthesis of primarily two proteins in a wheat germ cell-free system. Analysis of wheat germ cell-free products by sodium dodecyl sulfate-slab gel electrophoresis, direct immunoprecipitation with prolactin aatibody, and prolactin- and growth hormone-specific radioimmunoassay indicated that the two proteins were pre-prolactin and pre-growth hormone. Agarose-urea-gel electrophoresis of poly(A)-containing RNA revealed the presence of a large symmetrical RNA peak with an estimated size of approximately 15 S. The RNA contained in this peak was eluted from the gel and translated in the wheat germ cell-free system. Analysis of the translation products by electrophoresis, direct immunoprecipitation, and radioimmunoassay resulted in the detection of only pre-prolactin. The extent of pre-prolactin mRNA purification was assessed by synthesizing DNA complementary to gel-purified pre-prolactin mRNA and then following the hybridization of this cDNA to gel purified pre-prolactin mRNA. The result of this hybridization is consistent with the cell-free translation studies, namely that pre-prolactin mRNA is the predominant mRNA component of pituitary poly(A)-containing RNA. | lld:pubmed |
| pubmed-article:762150 | pubmed:language | eng | lld:pubmed |
| pubmed-article:762150 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:762150 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:762150 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:762150 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:762150 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:762150 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:762150 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:762150 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:762150 | pubmed:author | pubmed-author:ConveyE MEM | lld:pubmed |
| pubmed-article:762150 | pubmed:author | pubmed-author:RottmanF MFM | lld:pubmed |
| pubmed-article:762150 | pubmed:author | pubmed-author:NilsonJ HJH | lld:pubmed |
| pubmed-article:762150 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:762150 | pubmed:day | 10 | lld:pubmed |
| pubmed-article:762150 | pubmed:volume | 254 | lld:pubmed |
| pubmed-article:762150 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:762150 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:762150 | pubmed:pagination | 1516-20 | lld:pubmed |
| pubmed-article:762150 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:762150 | pubmed:meshHeading | pubmed-meshheading:762150-A... | lld:pubmed |
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| pubmed-article:762150 | pubmed:meshHeading | pubmed-meshheading:762150-P... | lld:pubmed |
| pubmed-article:762150 | pubmed:year | 1979 | lld:pubmed |
| pubmed-article:762150 | pubmed:articleTitle | Purification of pre-prolactin mRNA from bovine anterior pituitary glands. | lld:pubmed |
| pubmed-article:762150 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:762150 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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