| pubmed-article:7595514 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7595514 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:7595514 | lifeskim:mentions | umls-concept:C0027819 | lld:lifeskim |
| pubmed-article:7595514 | lifeskim:mentions | umls-concept:C0031715 | lld:lifeskim |
| pubmed-article:7595514 | lifeskim:mentions | umls-concept:C0376525 | lld:lifeskim |
| pubmed-article:7595514 | lifeskim:mentions | umls-concept:C0597357 | lld:lifeskim |
| pubmed-article:7595514 | lifeskim:mentions | umls-concept:C0017082 | lld:lifeskim |
| pubmed-article:7595514 | lifeskim:mentions | umls-concept:C0018270 | lld:lifeskim |
| pubmed-article:7595514 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:7595514 | pubmed:dateCreated | 1995-12-4 | lld:pubmed |
| pubmed-article:7595514 | pubmed:abstractText | SH-SY5Y is a thrice cloned cell line originally derived from the human neuroblastoma cell line SK-N-SH. It grows well in serum-containing medium and undergoes neuritogenesis in response to several trophic factors. Because it has been reported that this clonal line does not have receptors for platelet-derived growth factor (PDGF), it has been unclear what the major mitogenic factor in serum is for these cells. In competitive binding studies using radiolabeled PDGF-BB, we found that SH-SY5Y cells specifically bind PDGF with a KD = 0.14 +/- 0.06 nM and Bmax = 7.3 +/- 2.3 pM. Functionality of these receptors was demonstrated by an increased [3H]-thymidine incorporation in response to PDGF (stimulation index = 2.5). At concentrations of PDGF-BB between 5 and 100 ng/ml, maximum stimulation occurred with 20 ng/ml. Maximum DNA synthesis occurred after 12-24-h exposure to PDGF. Gangliosides GM3 and GT1b greatly inhibited [3H]thymidine incorporation, which was also inhibited to a lesser extent by GM1. Phosphorylation on tyrosine of a 170-kDa protein in response to PDGF stimulation of intact cells was demonstrated by western blot analysis probing with anti-phosphotyrosine antibody. Immunoprecipitation with anti-PDGF beta-receptor antibody and visualization on a western blot with an anti-phosphotyrosine antibody also revealed a 170-kDa protein. Maximum phosphorylation of the 170-kDa protein occurred after 5-min exposure to 20 ng/ml PDGF. This phosphorylation was inhibited by gangliosides GM1, GM2, GD1a, and GT1b but not by GM3. Receptor dimerization was also inhibited by GM1. These results show that SH-SY5Y cells have specific receptors for PDGF-BB that are functional, and can be modulated by gangliosides. | lld:pubmed |
| pubmed-article:7595514 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7595514 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7595514 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7595514 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7595514 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7595514 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7595514 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7595514 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7595514 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7595514 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7595514 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7595514 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7595514 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:7595514 | pubmed:issn | 0022-3042 | lld:pubmed |
| pubmed-article:7595514 | pubmed:author | pubmed-author:O'DorisioM... | lld:pubmed |
| pubmed-article:7595514 | pubmed:author | pubmed-author:YatesA JAJ | lld:pubmed |
| pubmed-article:7595514 | pubmed:author | pubmed-author:SummersMM | lld:pubmed |
| pubmed-article:7595514 | pubmed:author | pubmed-author:Van... | lld:pubmed |
| pubmed-article:7595514 | pubmed:author | pubmed-author:HyndsD LDL | lld:pubmed |
| pubmed-article:7595514 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7595514 | pubmed:volume | 65 | lld:pubmed |
| pubmed-article:7595514 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7595514 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7595514 | pubmed:pagination | 2251-8 | lld:pubmed |
| pubmed-article:7595514 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:7595514 | pubmed:meshHeading | pubmed-meshheading:7595514-... | lld:pubmed |
| pubmed-article:7595514 | pubmed:meshHeading | pubmed-meshheading:7595514-... | lld:pubmed |
| pubmed-article:7595514 | pubmed:meshHeading | pubmed-meshheading:7595514-... | lld:pubmed |
| pubmed-article:7595514 | pubmed:meshHeading | pubmed-meshheading:7595514-... | lld:pubmed |
| pubmed-article:7595514 | pubmed:meshHeading | pubmed-meshheading:7595514-... | lld:pubmed |
| pubmed-article:7595514 | pubmed:meshHeading | pubmed-meshheading:7595514-... | lld:pubmed |
| pubmed-article:7595514 | pubmed:meshHeading | pubmed-meshheading:7595514-... | lld:pubmed |
| pubmed-article:7595514 | pubmed:meshHeading | pubmed-meshheading:7595514-... | lld:pubmed |
| pubmed-article:7595514 | pubmed:meshHeading | pubmed-meshheading:7595514-... | lld:pubmed |
| pubmed-article:7595514 | pubmed:meshHeading | pubmed-meshheading:7595514-... | lld:pubmed |
| pubmed-article:7595514 | pubmed:meshHeading | pubmed-meshheading:7595514-... | lld:pubmed |
| pubmed-article:7595514 | pubmed:meshHeading | pubmed-meshheading:7595514-... | lld:pubmed |
| pubmed-article:7595514 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:7595514 | pubmed:articleTitle | Gangliosides inhibit platelet-derived growth factor-stimulated growth, receptor phosphorylation, and dimerization in neuroblastoma SH-SY5Y cells. | lld:pubmed |
| pubmed-article:7595514 | pubmed:affiliation | Department of Pathology (Neuropathology), Ohio State University, Columbus, USA. | lld:pubmed |
| pubmed-article:7595514 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7595514 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7595514 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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