| pubmed-article:7575451 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7575451 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:7575451 | lifeskim:mentions | umls-concept:C0086045 | lld:lifeskim |
| pubmed-article:7575451 | lifeskim:mentions | umls-concept:C0032433 | lld:lifeskim |
| pubmed-article:7575451 | lifeskim:mentions | umls-concept:C0443146 | lld:lifeskim |
| pubmed-article:7575451 | lifeskim:mentions | umls-concept:C0332281 | lld:lifeskim |
| pubmed-article:7575451 | lifeskim:mentions | umls-concept:C0205217 | lld:lifeskim |
| pubmed-article:7575451 | lifeskim:mentions | umls-concept:C1704259 | lld:lifeskim |
| pubmed-article:7575451 | lifeskim:mentions | umls-concept:C1705987 | lld:lifeskim |
| pubmed-article:7575451 | pubmed:dateCreated | 1995-11-9 | lld:pubmed |
| pubmed-article:7575451 | pubmed:abstractText | We previously reported that difluoromethylornithine (DFMO), an inhibitor of polyamine biosynthesis, exerted significant beneficial effects on the lifespan and disease expression of MRL-lpr/lpr mice, which spontaneously develop a lupus-like syndrome. Polyamine levels in splenic T-cells of MRL-lpr/lpr mice were significantly higher than those of Balb/c mice. In the present investigation, we examined the role of endogenous polyamines in transmembrane Ca2+ influx, generation of InsP3 and tyrosine phosphorylation of the p56lck protein in concanavalin A-stimulated splenic T-cells. Cytosolic free calcium concentrations ([Ca2+]i) in concanavalin A-stimulated T-cells of MRL-lpr/lpr and Balb/c mice were 250 +/- 25 and 450 +/- 42 nM respectively. Treatment of MRL-lpr/lpr mice with DFMO increased [Ca2+]i to 360 +/- 30 nM (P < 0.05). InsP3 levels of concanavalin A-stimulated MRL-lpr/lpr splenic T-cells were only 20% higher than those of unstimulated controls, whereas those of Balb/c T-cells were 90% higher. DFMO treatment increased InsP3 levels in concanavalin A-treated MRL-lpr/lpr T-cells to 67%. Western-blot analysis showed a 7-fold higher level of p56lck phosphorylation of MRL-lpr/lpr splenic T-cells than that of Balb/c mice. DFMO treatment reduced tyrosine phosphorylation of p56lck of MRL-lpr/lpr mice significantly (P < 0.001). Two-colour flow-cytometric analysis revealed no significant difference in the CD4+/CD8+ ratio in splenic T-cells of MRL-lpr/lpr mice after DFMO treatment. Polyamine levels in splenocytes were significantly reduced by DFMO treatment. These data show that DFMO treatment could alter signal-transduction pathways of splenic T-cells of MRL-lpr/lpr mice. Increased levels of polyamines in T-cells of untreated lpr mice contribute to defective signal-transduction pathways and the pathogenesis of lupus-like symptoms. | lld:pubmed |
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| pubmed-article:7575451 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7575451 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7575451 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7575451 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:7575451 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7575451 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:7575451 | pubmed:issn | 0264-6021 | lld:pubmed |
| pubmed-article:7575451 | pubmed:author | pubmed-author:ThomasTT | lld:pubmed |
| pubmed-article:7575451 | pubmed:author | pubmed-author:ThomasT JTJ | lld:pubmed |
| pubmed-article:7575451 | pubmed:author | pubmed-author:SeiboldJ RJR | lld:pubmed |
| pubmed-article:7575451 | pubmed:author | pubmed-author:GunniaU BUB | lld:pubmed |
| pubmed-article:7575451 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7575451 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:7575451 | pubmed:volume | 311 ( Pt 1) | lld:pubmed |
| pubmed-article:7575451 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7575451 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7575451 | pubmed:pagination | 175-82 | lld:pubmed |
| pubmed-article:7575451 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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