| pubmed-article:7559806 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7559806 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:7559806 | lifeskim:mentions | umls-concept:C0225335 | lld:lifeskim |
| pubmed-article:7559806 | lifeskim:mentions | umls-concept:C0033689 | lld:lifeskim |
| pubmed-article:7559806 | lifeskim:mentions | umls-concept:C0205307 | lld:lifeskim |
| pubmed-article:7559806 | lifeskim:mentions | umls-concept:C0031164 | lld:lifeskim |
| pubmed-article:7559806 | lifeskim:mentions | umls-concept:C1705944 | lld:lifeskim |
| pubmed-article:7559806 | lifeskim:mentions | umls-concept:C1522408 | lld:lifeskim |
| pubmed-article:7559806 | lifeskim:mentions | umls-concept:C0453946 | lld:lifeskim |
| pubmed-article:7559806 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:7559806 | pubmed:dateCreated | 1995-10-31 | lld:pubmed |
| pubmed-article:7559806 | pubmed:abstractText | The composition and permeability of the pericellular coat surrounding normal human mesothelial (NHM) cells have been studied in vitro. NHM cells were grown in the presence of 3H-glucosamine and the amount of label recovered in hyaluronan and chondroitin sulfate was determined after selective enzymatic digestion of the polysaccharides in medium, pericellular, and intracellular pools. For comparison a similar analysis was carried out on mesothelioma cells (Mero-14). Of the labeled polysaccharides in the medium and pericellular pools of NHM cells about 80-90% could be ascribed to hyaluronan and only 3-5% to chondroitin sulfate. In contrast, Mero-14 synthesized only minute amounts of hyaluronan whereas chondroitin sulfate corresponded to 61% of the total glycosaminoglycans in the culture. The results exclude a structure of the pericellular layer of NHM cells similar to the hyaluronan-proteoglycan aggregates found in cartilage. The permeability of the pericellular layer was tested by the exclusion of polystyrene microspheres and bacteria of diameter 0.1-3.0 microns, as well as erythrocytes of diameter 7 microns. While the erythrocytes were excluded the smaller particles penetrated the coat. By adding 0.5 mg/ml of aggregating cartilage proteoglycan to the medium particles of 0.3 microns or larger were also excluded. Thus exogenous proteoglycans can reinforce the structure of the pericellular layer. | lld:pubmed |
| pubmed-article:7559806 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7559806 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7559806 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7559806 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7559806 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7559806 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7559806 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:7559806 | pubmed:issn | 0021-9541 | lld:pubmed |
| pubmed-article:7559806 | pubmed:author | pubmed-author:SuzukiMM | lld:pubmed |
| pubmed-article:7559806 | pubmed:author | pubmed-author:PertoftHH | lld:pubmed |
| pubmed-article:7559806 | pubmed:author | pubmed-author:TederPP | lld:pubmed |
| pubmed-article:7559806 | pubmed:author | pubmed-author:HeldinPP | lld:pubmed |
| pubmed-article:7559806 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7559806 | pubmed:volume | 165 | lld:pubmed |
| pubmed-article:7559806 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7559806 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7559806 | pubmed:pagination | 54-61 | lld:pubmed |
| pubmed-article:7559806 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
| pubmed-article:7559806 | pubmed:meshHeading | pubmed-meshheading:7559806-... | lld:pubmed |
| pubmed-article:7559806 | pubmed:meshHeading | pubmed-meshheading:7559806-... | lld:pubmed |
| pubmed-article:7559806 | pubmed:meshHeading | pubmed-meshheading:7559806-... | lld:pubmed |
| pubmed-article:7559806 | pubmed:meshHeading | pubmed-meshheading:7559806-... | lld:pubmed |
| pubmed-article:7559806 | pubmed:meshHeading | pubmed-meshheading:7559806-... | lld:pubmed |
| pubmed-article:7559806 | pubmed:meshHeading | pubmed-meshheading:7559806-... | lld:pubmed |
| pubmed-article:7559806 | pubmed:meshHeading | pubmed-meshheading:7559806-... | lld:pubmed |
| pubmed-article:7559806 | pubmed:meshHeading | pubmed-meshheading:7559806-... | lld:pubmed |
| pubmed-article:7559806 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:7559806 | pubmed:articleTitle | Chondroitin sulfate proteoglycan modulates the permeability of hyaluronan-containing coats around normal human mesothelial cells. | lld:pubmed |
| pubmed-article:7559806 | pubmed:affiliation | Department of Medical and Physiological Chemistry, University of Uppsala, Sweden. | lld:pubmed |
| pubmed-article:7559806 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7559806 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:7559806 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7559806 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7559806 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7559806 | lld:pubmed |
