| pubmed-article:7536457 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7536457 | lifeskim:mentions | umls-concept:C0206655 | lld:lifeskim |
| pubmed-article:7536457 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:7536457 | lifeskim:mentions | umls-concept:C1515752 | lld:lifeskim |
| pubmed-article:7536457 | lifeskim:mentions | umls-concept:C0332466 | lld:lifeskim |
| pubmed-article:7536457 | lifeskim:mentions | umls-concept:C0162789 | lld:lifeskim |
| pubmed-article:7536457 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:7536457 | pubmed:dateCreated | 1995-5-23 | lld:pubmed |
| pubmed-article:7536457 | pubmed:abstractText | Cytogenetic studies of the pediatric solid tumor alveolar rhabdomyosarcoma have demonstrated the presence of a consistent chromosomal translocation, t(2;13)(q35;q14). We recently identified PAX3 and FKHR as the genes on chromosomes 2 and 13, respectively, that are juxtaposed by this translocation. As one means of detecting the t(2;13) translocation in clinical specimens, we have developed a fluorescence in situ hybridization (FISH) assay that may be used for both interphase and metaphase cells. Translocation of the 5' region of the FKHR gene to the derivative chromosome 2, and retention of the 3' region of FKHR on the derivative chromosome 13 [(der(13)], were demonstrated in metaphase cells from a rhabdomyosarcoma cell line with a previously identified t(2;13) translocation. A 5' PAX3 cosmid probe was shown to localize to 2q35 in normal cells, and to translocate to the der(13) in the rhabdomyosarcoma cell line. In order to detect the der(13) in interphase nuclei, we labeled the 3'FKHR and the 5'PAX3 cosmid probes with digoxigenin and biotin, respectively, and used these in a two-color FISH assay. The presence of the der(13) was visualized as juxtaposed or overlapping red and green signals in metaphase and interphase tumor cells. The PAX3-FKHR FISH assay was then applied to a series of cytogenetically characterized pediatric sarcoma cell lines. The presence of the der(13) was demonstrated by FISH in all cases containing a cytogenetically detectable t(2;13). The FISH assay was then applied to a series of 20 embryonal and alveolar rhabdomyosarcoma samples. All 10 of the alveolar rhabdomyosarcoma specimens demonstrated a der(13) with the FISH assay.(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
| pubmed-article:7536457 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7536457 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7536457 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7536457 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7536457 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7536457 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7536457 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7536457 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:7536457 | pubmed:issn | 1045-2257 | lld:pubmed |
| pubmed-article:7536457 | pubmed:author | pubmed-author:ShapiroD NDN | lld:pubmed |
| pubmed-article:7536457 | pubmed:author | pubmed-author:BiegelJ AJA | lld:pubmed |
| pubmed-article:7536457 | pubmed:author | pubmed-author:BarrF GFG | lld:pubmed |
| pubmed-article:7536457 | pubmed:author | pubmed-author:NycumL MLM | lld:pubmed |
| pubmed-article:7536457 | pubmed:author | pubmed-author:ValentineVV | lld:pubmed |
| pubmed-article:7536457 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7536457 | pubmed:volume | 12 | lld:pubmed |
| pubmed-article:7536457 | pubmed:geneSymbol | FKHR | lld:pubmed |
| pubmed-article:7536457 | pubmed:geneSymbol | PAX3 | lld:pubmed |
| pubmed-article:7536457 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7536457 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7536457 | pubmed:pagination | 186-92 | lld:pubmed |
| pubmed-article:7536457 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:7536457 | pubmed:meshHeading | pubmed-meshheading:7536457-... | lld:pubmed |
| pubmed-article:7536457 | pubmed:meshHeading | pubmed-meshheading:7536457-... | lld:pubmed |
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| pubmed-article:7536457 | pubmed:meshHeading | pubmed-meshheading:7536457-... | lld:pubmed |
| pubmed-article:7536457 | pubmed:meshHeading | pubmed-meshheading:7536457-... | lld:pubmed |
| pubmed-article:7536457 | pubmed:meshHeading | pubmed-meshheading:7536457-... | lld:pubmed |
| pubmed-article:7536457 | pubmed:meshHeading | pubmed-meshheading:7536457-... | lld:pubmed |
| pubmed-article:7536457 | pubmed:meshHeading | pubmed-meshheading:7536457-... | lld:pubmed |
| pubmed-article:7536457 | pubmed:meshHeading | pubmed-meshheading:7536457-... | lld:pubmed |
| pubmed-article:7536457 | pubmed:meshHeading | pubmed-meshheading:7536457-... | lld:pubmed |
| pubmed-article:7536457 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:7536457 | pubmed:articleTitle | Detection of the t(2;13)(q35;q14) and PAX3-FKHR fusion in alveolar rhabdomyosarcoma by fluorescence in situ hybridization. | lld:pubmed |
| pubmed-article:7536457 | pubmed:affiliation | Division of Human Genetics and Molecular Biology, Children's Hospital of Philadelphia, PA 19104, USA. | lld:pubmed |
| pubmed-article:7536457 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7536457 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7536457 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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