| pubmed-article:7518821 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7518821 | lifeskim:mentions | umls-concept:C0014597 | lld:lifeskim |
| pubmed-article:7518821 | lifeskim:mentions | umls-concept:C0242275 | lld:lifeskim |
| pubmed-article:7518821 | lifeskim:mentions | umls-concept:C0205064 | lld:lifeskim |
| pubmed-article:7518821 | lifeskim:mentions | umls-concept:C0040845 | lld:lifeskim |
| pubmed-article:7518821 | lifeskim:mentions | umls-concept:C0441889 | lld:lifeskim |
| pubmed-article:7518821 | lifeskim:mentions | umls-concept:C0021665 | lld:lifeskim |
| pubmed-article:7518821 | lifeskim:mentions | umls-concept:C0242210 | lld:lifeskim |
| pubmed-article:7518821 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:7518821 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:7518821 | pubmed:dateCreated | 1994-8-25 | lld:pubmed |
| pubmed-article:7518821 | pubmed:abstractText | Insulin-like growth factors (IGFs) are important regulators of epithelial cell growth. The mitogenic activity of these factors is influenced by the levels of extracellular IGF binding proteins, including insulin-like growth factor binding protein 3 (IGFBP-3). In the present report we study the effects of epidermal growth factor (EGF) and all-trans-retinoic acid (RA) on IGFBP-3 RNA and protein levels in human papillomavirus-immortalized cervical epithelial cells. Treatment of ECE16-1 cells with 3-20 ng/ml EGF causes a marked reduction in IGFBP-3 levels. In contrast, 1 microM RA increases IGFBP-3 mRNA and protein levels in the presence or absence of 20 ng/ml EGF. The response is concentration dependent with a half-maximal increase observed at 1 nM RA. RA is able to reverse the EGF suppression when added simultaneously or 3 days after initiation of EGF treatment. Conversely, when cells are treated with RA, IGFBP-3 levels increase within 24 h and subsequent addition of EGF is without effect. Thus, the RA-dependent increase in IGFBP-3 levels is dominant over the EGF suppression. The increased IGFBP-3 levels are correlated with RA suppression of proliferation. Similar RA effects on IGFBP-3 mRNA levels were observed in other cervical epithelial cell lines (i.e., ECE16-D1, ECE16-D2, and CaSki). These results suggest that RA may act to inhibit cervical cell growth by increasing IGFBP-3 levels and reducing the extracellular concentration of free insulin-like growth factor I (IGFI) and/or alternatively, IGFBP-3 may inhibit cell growth by direct effects on the cell, independent of IGFI. | lld:pubmed |
| pubmed-article:7518821 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7518821 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7518821 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7518821 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7518821 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7518821 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7518821 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7518821 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7518821 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7518821 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7518821 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:7518821 | pubmed:issn | 0021-9541 | lld:pubmed |
| pubmed-article:7518821 | pubmed:author | pubmed-author:EckertR LRL | lld:pubmed |
| pubmed-article:7518821 | pubmed:author | pubmed-author:HembreeJ RJR | lld:pubmed |
| pubmed-article:7518821 | pubmed:author | pubmed-author:Andreatta-Van... | lld:pubmed |
| pubmed-article:7518821 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7518821 | pubmed:volume | 160 | lld:pubmed |
| pubmed-article:7518821 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7518821 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7518821 | pubmed:pagination | 265-74 | lld:pubmed |
| pubmed-article:7518821 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:7518821 | pubmed:meshHeading | pubmed-meshheading:7518821-... | lld:pubmed |
| pubmed-article:7518821 | pubmed:meshHeading | pubmed-meshheading:7518821-... | lld:pubmed |
| pubmed-article:7518821 | pubmed:meshHeading | pubmed-meshheading:7518821-... | lld:pubmed |
| pubmed-article:7518821 | pubmed:meshHeading | pubmed-meshheading:7518821-... | lld:pubmed |
| pubmed-article:7518821 | pubmed:meshHeading | pubmed-meshheading:7518821-... | lld:pubmed |
| pubmed-article:7518821 | pubmed:meshHeading | pubmed-meshheading:7518821-... | lld:pubmed |
| pubmed-article:7518821 | pubmed:meshHeading | pubmed-meshheading:7518821-... | lld:pubmed |
| pubmed-article:7518821 | pubmed:meshHeading | pubmed-meshheading:7518821-... | lld:pubmed |
| pubmed-article:7518821 | pubmed:meshHeading | pubmed-meshheading:7518821-... | lld:pubmed |
| pubmed-article:7518821 | pubmed:meshHeading | pubmed-meshheading:7518821-... | lld:pubmed |
| pubmed-article:7518821 | pubmed:meshHeading | pubmed-meshheading:7518821-... | lld:pubmed |
| pubmed-article:7518821 | pubmed:meshHeading | pubmed-meshheading:7518821-... | lld:pubmed |
| pubmed-article:7518821 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7518821 | pubmed:articleTitle | Regulation of insulin-like growth factor 1 binding protein 3 levels by epidermal growth factor and retinoic acid in cervical epithelial cells. | lld:pubmed |
| pubmed-article:7518821 | pubmed:affiliation | Department of Physiology, Case Western Reserve University, School of Medicine, Cleveland, Ohio 44106-4970. | lld:pubmed |
| pubmed-article:7518821 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7518821 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7518821 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7518821 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7518821 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7518821 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7518821 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7518821 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7518821 | lld:pubmed |
