| pubmed-article:725798 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:725798 | lifeskim:mentions | umls-concept:C1330957 | lld:lifeskim |
| pubmed-article:725798 | lifeskim:mentions | umls-concept:C0596311 | lld:lifeskim |
| pubmed-article:725798 | lifeskim:mentions | umls-concept:C0563594 | lld:lifeskim |
| pubmed-article:725798 | lifeskim:mentions | umls-concept:C0175668 | lld:lifeskim |
| pubmed-article:725798 | lifeskim:mentions | umls-concept:C2825348 | lld:lifeskim |
| pubmed-article:725798 | lifeskim:mentions | umls-concept:C0205225 | lld:lifeskim |
| pubmed-article:725798 | pubmed:dateCreated | 1979-2-23 | lld:pubmed |
| pubmed-article:725798 | pubmed:abstractText | Previously it was shown that the tryptic digestion of human IgM at 65 C yield Fc'mu fragments in addition to Fabmu and (Fc)5mu fragments. This Fc'mu was found to be derived from the Cmu4 domain of the mu-chain. Additional studies were done on the effects of tryptic digestion time and temperature on the proteolytic process of IgM, IgM digestion by 2% trypsin at 65 C produced (Fc)8mu and Fc'mu in approximately a 3:2 ratio. While an appreciable amount of intact (Fc)5mu was present along with Fc'mu in the IgM digest, no residual "(Fc)5mu fragment" with its C-terminal segment missing was found. If the temperature of digestion is held constant at either 56 C or 60 C and the digestion time is varied from 20 to 90 min, there is also progressive cleavage of IgM with a concomitant increase in the yield of Fc'mu. It appears that the tryptic digestion of IgM is a stepwise process and that the primary cleavage of IgM occurs at Arg-325(2) of all ten mu-chains and the (Fc)5mu is subsequently degraded to Fc'mu fragments by secondary cleavage. | lld:pubmed |
| pubmed-article:725798 | pubmed:language | eng | lld:pubmed |
| pubmed-article:725798 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:725798 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:725798 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:725798 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:725798 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:725798 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:725798 | pubmed:issn | 0040-4675 | lld:pubmed |
| pubmed-article:725798 | pubmed:author | pubmed-author:ChenJ PJP | lld:pubmed |
| pubmed-article:725798 | pubmed:author | pubmed-author:KalmazE VEV | lld:pubmed |
| pubmed-article:725798 | pubmed:author | pubmed-author:ElakovichS... | lld:pubmed |
| pubmed-article:725798 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:725798 | pubmed:volume | 36 | lld:pubmed |
| pubmed-article:725798 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:725798 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:725798 | pubmed:pagination | 79-93 | lld:pubmed |
| pubmed-article:725798 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:725798 | pubmed:meshHeading | pubmed-meshheading:725798-H... | lld:pubmed |
| pubmed-article:725798 | pubmed:meshHeading | pubmed-meshheading:725798-H... | lld:pubmed |
| pubmed-article:725798 | pubmed:meshHeading | pubmed-meshheading:725798-O... | lld:pubmed |
| pubmed-article:725798 | pubmed:meshHeading | pubmed-meshheading:725798-T... | lld:pubmed |
| pubmed-article:725798 | pubmed:meshHeading | pubmed-meshheading:725798-T... | lld:pubmed |
| pubmed-article:725798 | pubmed:meshHeading | pubmed-meshheading:725798-A... | lld:pubmed |
| pubmed-article:725798 | pubmed:meshHeading | pubmed-meshheading:725798-A... | lld:pubmed |
| pubmed-article:725798 | pubmed:meshHeading | pubmed-meshheading:725798-C... | lld:pubmed |
| pubmed-article:725798 | pubmed:meshHeading | pubmed-meshheading:725798-I... | lld:pubmed |
| pubmed-article:725798 | pubmed:meshHeading | pubmed-meshheading:725798-E... | lld:pubmed |
| pubmed-article:725798 | pubmed:meshHeading | pubmed-meshheading:725798-I... | lld:pubmed |
| pubmed-article:725798 | pubmed:year | 1978 | lld:pubmed |
| pubmed-article:725798 | pubmed:articleTitle | Primary and secondary tryptic cleavages of human IgM at high temperature. | lld:pubmed |
| pubmed-article:725798 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:725798 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
