| pubmed-article:7108231 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7108231 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:7108231 | lifeskim:mentions | umls-concept:C0003320 | lld:lifeskim |
| pubmed-article:7108231 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:7108231 | lifeskim:mentions | umls-concept:C0600190 | lld:lifeskim |
| pubmed-article:7108231 | lifeskim:mentions | umls-concept:C1419917 | lld:lifeskim |
| pubmed-article:7108231 | lifeskim:mentions | umls-concept:C1305923 | lld:lifeskim |
| pubmed-article:7108231 | lifeskim:mentions | umls-concept:C1705822 | lld:lifeskim |
| pubmed-article:7108231 | lifeskim:mentions | umls-concept:C0348011 | lld:lifeskim |
| pubmed-article:7108231 | lifeskim:mentions | umls-concept:C0183235 | lld:lifeskim |
| pubmed-article:7108231 | lifeskim:mentions | umls-concept:C0392762 | lld:lifeskim |
| pubmed-article:7108231 | lifeskim:mentions | umls-concept:C0071500 | lld:lifeskim |
| pubmed-article:7108231 | lifeskim:mentions | umls-concept:C0439643 | lld:lifeskim |
| pubmed-article:7108231 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:7108231 | pubmed:dateCreated | 1982-10-21 | lld:pubmed |
| pubmed-article:7108231 | pubmed:abstractText | Polypeptides (233 micrograms) from brain synaptosomes resolved in SDS 7.5%--15% polyacrylamide gradient gels were electrophoretically transferred quantitatively to nitrocellulose sheets at 3 V/cm for 21 h in the following buffer: 25 mM Tris-192 mM glycine, pH 8.3/20% methanol/0.1% SDS. After immunoautoradiographic detection of antigens on this nitrocellulose replica with either rabbit anti-rat cerebrum immunoglobulins or a mouse monoclonal antibody to a rat synaptosomal protein, the antibody and [125I]protein A were removed from the replica by treatment with 8 M urea, 0.1 M 2-mercaptoethanol, and 5 mg/ml BSA at 60 degrees C for 1 h. The replicas were successfully re-used by re-exposing them to the antibody and [125I]protein A. | lld:pubmed |
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| pubmed-article:7108231 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7108231 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:7108231 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:7108231 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7108231 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:7108231 | pubmed:issn | 0022-1759 | lld:pubmed |
| pubmed-article:7108231 | pubmed:author | pubmed-author:LasherR SRS | lld:pubmed |
| pubmed-article:7108231 | pubmed:author | pubmed-author:MinierL NLN | lld:pubmed |
| pubmed-article:7108231 | pubmed:author | pubmed-author:EricksonP FPF | lld:pubmed |
| pubmed-article:7108231 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7108231 | pubmed:day | 11 | lld:pubmed |
| pubmed-article:7108231 | pubmed:volume | 51 | lld:pubmed |
| pubmed-article:7108231 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7108231 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7108231 | pubmed:pagination | 241-9 | lld:pubmed |
| pubmed-article:7108231 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:7108231 | pubmed:year | 1982 | lld:pubmed |
| pubmed-article:7108231 | pubmed:articleTitle | Quantitative electrophoretic transfer of polypeptides from SDS polyacrylamide gels to nitrocellulose sheets: a method for their re-use in immunoautoradiographic detection of antigens. | lld:pubmed |
| pubmed-article:7108231 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7108231 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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