| pubmed-article:7021150 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7021150 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:7021150 | lifeskim:mentions | umls-concept:C0030016 | lld:lifeskim |
| pubmed-article:7021150 | lifeskim:mentions | umls-concept:C0443286 | lld:lifeskim |
| pubmed-article:7021150 | lifeskim:mentions | umls-concept:C0750729 | lld:lifeskim |
| pubmed-article:7021150 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:7021150 | pubmed:dateCreated | 1981-10-29 | lld:pubmed |
| pubmed-article:7021150 | pubmed:abstractText | The steric course of the reaction catalyzed by Escherichia coli enoyl acyl-carrier-protein reductase was studied. 1. trans-2-[2-2H1]Decenoic and trans-2-[3-2H1]decenoic acids were synthesized and converted to the corresponding decenoyl thiol esters with CoA or acyl carrier protein. These deuterium-labeled decenoyl thiol esters were incubated with purified enoyl acyl-carrier-protein reductase in the presence of NADPH or NADH. 2. The unlabeled trans-2-decenoyl thiol esters were incubated with enoyl acyl-carrier-protein reductase in the presence of (4S)-[4-2H1]NADH. The unlabeled decenoyl thiol esters were also incubated with the enzyme in 2H2O. The decanoic acids formed in the above incubations were extracted and subjected to the action of acyl-CoA oxidase, which had been previously shown to catalyze the anti elimination of the pro-2R and pro-3R hydrogens of acyl-CoA. The resulting products, 2-decenoyl-CoAs, were converted to methyl esters and their deuterium contents were analyzed by gas chromatography/mass spectrometry. The results suggested that the reduction catalyzed by E. coli enoyl acyl-carrier-protein reductase occurs by a syn addition of hydrogen via a 2-Re, 3-Si attack on the double bond. | lld:pubmed |
| pubmed-article:7021150 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7021150 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7021150 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7021150 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7021150 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7021150 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7021150 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7021150 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7021150 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7021150 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7021150 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7021150 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:7021150 | pubmed:issn | 0014-2956 | lld:pubmed |
| pubmed-article:7021150 | pubmed:author | pubmed-author:SaitoKK | lld:pubmed |
| pubmed-article:7021150 | pubmed:author | pubmed-author:OkudaSS | lld:pubmed |
| pubmed-article:7021150 | pubmed:author | pubmed-author:YamakawaTT | lld:pubmed |
| pubmed-article:7021150 | pubmed:author | pubmed-author:KawaguchiAA | lld:pubmed |
| pubmed-article:7021150 | pubmed:author | pubmed-author:SeyamaYY | lld:pubmed |
| pubmed-article:7021150 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7021150 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:7021150 | pubmed:volume | 116 | lld:pubmed |
| pubmed-article:7021150 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7021150 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7021150 | pubmed:pagination | 581-6 | lld:pubmed |
| pubmed-article:7021150 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:7021150 | pubmed:meshHeading | pubmed-meshheading:7021150-... | lld:pubmed |
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| pubmed-article:7021150 | pubmed:meshHeading | pubmed-meshheading:7021150-... | lld:pubmed |
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| pubmed-article:7021150 | pubmed:meshHeading | pubmed-meshheading:7021150-... | lld:pubmed |
| pubmed-article:7021150 | pubmed:meshHeading | pubmed-meshheading:7021150-... | lld:pubmed |
| pubmed-article:7021150 | pubmed:meshHeading | pubmed-meshheading:7021150-... | lld:pubmed |
| pubmed-article:7021150 | pubmed:meshHeading | pubmed-meshheading:7021150-... | lld:pubmed |
| pubmed-article:7021150 | pubmed:meshHeading | pubmed-meshheading:7021150-... | lld:pubmed |
| pubmed-article:7021150 | pubmed:year | 1981 | lld:pubmed |
| pubmed-article:7021150 | pubmed:articleTitle | Steric course of reaction catalyzed by the enoyl acyl-carrier-protein reductase of Escherichia coli. | lld:pubmed |
| pubmed-article:7021150 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7021150 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7021150 | lld:pubmed |
